Anti-DDR2抗体[3B11E4] (ab63337)
![Western blot - Anti-DDR2 antibody [3B11E4] (ab63337)](https://www.abcam.cn/ps/products/63/ab63337/Images/ab63337wb.jpg "Western blot - Anti-DDR2 antibody [3B11E4] (ab63337)")
Key features and details
- Mouse monoclonal [3B11E4] to DDR2
- Suitable for: Flow Cyt, WB, ICC/IF, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
概述
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产品名称
Anti-DDR2抗体[3B11E4]
参阅全部 DDR2 一抗 -
描述
小鼠单克隆抗体[3B11E4] to DDR2 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human DDR2. Purified truncated recombinant Human DDR2 expressed in E. Coli strain BL21 (DE3)
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阳性对照
- ICC/IF: A549 cells Flow Cyt: A549 cells Gc-7901 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
3B11E4 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63337于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/100 - 1/200.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| WB |
1/500 - 1/2000. Predicted molecular weight: 97 kDa.
|
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| ICC/IF | (1) |
1/500 - 1/2000.
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| IHC-P | (1) |
1/500 - 1/2000.
|
| 说明 |
|---|
|
Flow Cyt
1/100 - 1/200. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
WB
1/500 - 1/2000. Predicted molecular weight: 97 kDa. |
|
ICC/IF
1/500 - 1/2000. |
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IHC-P
1/500 - 1/2000. |
靶标
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功能
This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. -
组织特异性
The major 10 kDa transcript is expressed in high levels in heart and lung, less in brain, placenta, liver, skeletal muscle, pancreas, and kidney. -
疾病相关
Defects in DDR2 are the cause of spondyloepimetaphyseal dysplasia short limb-hand type (SEMD-SL) [MIM:271665]. A bone disease characterized by short-limbed dwarfism, a narrow chest with pectus excavatum, brachydactyly in the hands and feet, a characteristic craniofacial appearance and premature calcifications. The radiological findings are distinctive and comprise short long bones throughout the skeleton with striking epiphyses that are stippled, flattened and fragmented and flared, irregular metaphyses. Platyspondyly in the spine with wide intervertebral spaces is observed and some vertebral bodies are pear-shaped with central humps, anterior protrusions and posterior scalloping. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 F5/8 type C domain.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4921 Human
- Omim: 191311 Human
- SwissProt: Q16832 Human
- Unigene: 275757 Human
- Unigene: 593833 Human
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别名
- CD167 antigen-like family member B antibody
- CD167b antibody
- CD167b antigen antibody
see all
图片
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Western blot - Anti-DDR2 antibody [3B11E4] (ab63337)All lanes : Anti-DDR2 antibody [3B11E4] (ab63337) at 1/500 dilution
Lane 1 : molecular weight markers
Lane 2 : truncated recombinant DDR2 (immunizing peptide)
Predicted band size: 97 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody [3B11E4] (ab63337)](https://www.abcam.cn/ps/products/63/ab63337/Images/DDR2-Primary-antibodies-ab63337-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody [3B11E4] (ab63337)ab63337 at 1/1000 dilution staining DDR2 in human skeletal muscle tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections).
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![Immunocytochemistry/ Immunofluorescence - Anti-DDR2 antibody [3B11E4] (ab63337)](https://www.abcam.cn/ps/products/63/ab63337/Images/DDR2-Primary-antibodies-ab63337-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DDR2 antibody [3B11E4] (ab63337)ab63337 at 1/1000 dilution staining DDR2 in human A549 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® conjugated Goat anti-mouse was used as secondary. The actin filaments were labeled red with DY-554 phalloidin whilst nuclei were stained blue with DRAQ5 fluorescent DNA dye.
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![Flow Cytometry - Anti-DDR2 antibody [3B11E4] (ab63337)](https://www.abcam.cn/ps/products/63/ab63337/Images/ab63337-245731-ab633372.jpg)
Flow Cytometry - Anti-DDR2 antibody [3B11E4] (ab63337)Overlay histogram showing A549 cells stained with ab63337 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63337, 1/200 dilution) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) preadsorbed (ab150117) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a (ab170191, 0.5μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab63337 被引用在 6 文献中.
- Hao Y et al. Tumor elastography and its association with cell-free tumor DNA in the plasma of breast tumor patients: a pilot study. Quant Imaging Med Surg 11:3518-3534 (2021). PubMed: 34341728
- Mount S et al. Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium. Stem Cell Res Ther 10:316 (2019). PubMed: 31685023
- Ali II et al. Oxytocin induces intracellular Ca2+ release in cardiac fibroblasts from neonatal rats. Cell Calcium 84:102099 (2019). PubMed: 31614270
- Li D et al. Long Noncoding RNA pancEts-1 Promotes Neuroblastoma Progression through hnRNPK-Mediated ß-Catenin Stabilization. Cancer Res 78:1169-1183 (2018). PubMed: 29311158
- Skiöldebrand E et al. Inflammatory activation of human cardiac fibroblasts leads to altered calcium signaling, decreased connexin 43 expression and increased glutamate secretion. Heliyon 3:e00406 (2017). PubMed: 29022008
- Suutre S et al. Evaluation of correlation of articular cartilage staining for DDR2 and proteoglycans with histological tissue damage and the results of radiographic assessment in patients with early stages of knee osteoarthritis. Int J Clin Exp Pathol 8:5658-65 (2015). IHC . PubMed: 26191278