Anti-DDIT3 (phospho S30)抗体(ab63392)
Key features and details
- Rabbit polyclonal to DDIT3 (phospho S30)
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DDIT3 (phospho S30)抗体
参阅全部 DDIT3 一抗 -
描述
兔多克隆抗体to DDIT3 (phospho S30) -
宿主
Rabbit -
特异性
This antibody detects endogenous levels of GADD153 only when phosphorylated at serine 30. -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human DDIT3 (phospho S30).
Database link: P35638 -
阳性对照
- A549 cells and human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63392于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
|
|
| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
ICC/IF
1/500 - 1/1000. |
|
IHC-P
1/50 - 1/100. |
靶标
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功能
Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. -
疾病相关
Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS. -
序列相似性
Belongs to the bZIP family.
Contains 1 bZIP domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1649 Human
- Entrez Gene: 13198 Mouse
- Omim: 126337 Human
- SwissProt: P35638 Human
- SwissProt: P35639 Mouse
- Unigene: 505777 Human
- Unigene: 728989 Human
- Unigene: 110220 Mouse
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别名
- C/EBP homologous protein antibody
- C/EBP Homology Protein antibody
- C/EBP zeta antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-DDIT3 (phospho S30) antibody (ab63392)Immunofluorescence analysis of A549 cells, using ab63392 at 1:500 dilution.
Left image untreated.Right image treated with phospho peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDIT3 (phospho S30) antibody (ab63392)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab63392 at 1:50 dilution.
Left image untreated.Right image treated with phospho peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab63392 被引用在 1 文献中.
- Tian H et al. D4F alleviates the C/EBP homologous protein-mediated apoptosis in glycated high-density lipoprotein-treated macrophages by facilitating autophagy. Exp Biol Med (Maywood) 246:2595-2609 (2021). PubMed: 34525858