Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[FG4R] (ab125243)
![Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)](https://www.abcam.cn/ps/products/125/ab125243/Images/ab125243-139413-anti-ddddk-tag-antibody-fg4r-equivalent-to-flag-antibodies-from-sigma-western-blot.jpg "Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)")
Key features and details
- Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB, ELISA, Dot blot, IP, In situ hybridization
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[FG4R]
参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗 -
描述
小鼠单克隆抗体[FG4R] to DDDDK tag (Binds to FLAG® tag sequence) -
宿主
Mouse -
特异性
Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged (also known as the FLAG® tag sequence) fusion proteins.
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经测试应用
适用于: WB, ELISA, Dot blot, IP, In situ hybridizationmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
Sequence:DYKDDDDK
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阳性对照
- HEK293 cells transfected with DYKDDDDK (also known as the FLAG® tag sequence).
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常规说明
If stored at 4°C please add 0.1% sodium azide.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
ab125243 was purified by protein A affinity chromatography from TCS -
克隆
单克隆 -
克隆编号
FG4R -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125243于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/3000.
(with ECL) |
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| ELISA |
Use at an assay dependent concentration.
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| Dot blot |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| In situ hybridization |
1/500 - 1/2000.
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| 说明 |
|---|
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WB
1/1000 - 1/3000. (with ECL) |
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ELISA
Use at an assay dependent concentration. |
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Dot blot
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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In situ hybridization
1/500 - 1/2000. |
靶标
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相关性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
别名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
图片
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Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)All lanes : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243) at 1/1000 dilution
Lane 1 : HEK293 cell lysate non-transfected
Lane 2 : HEK293 cell lysate transfected with DDDDK tagged protein vector
Developed using the ECL technique.
数据表及文件
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SDS download
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Datasheet download
文献 (34)
ab125243 被引用在 34 文献中.
- Zhang H et al. Acetylation stabilizes the signaling protein WISP2 by preventing its degradation to suppress the progression of acute myeloid leukemia. J Biol Chem 299:102971 (2023). PubMed: 36736423
- Tao XY et al. Biotinylated Tn5 transposase-mediated CUT&Tag efficiently profiles transcription factor-DNA interactions in plants. Plant Biotechnol J 21:1191-1205 (2023). PubMed: 36786225
- Chen DD et al. Structure, catalysis, chitin transport, and selective inhibition of chitin synthase. Nat Commun 14:4776 (2023). PubMed: 37553334
- Li Y et al. Chidamide Suppresses the Growth of Cholangiocarcinoma by Inhibiting HDAC3 and Promoting FOXO1 Acetylation. Stem Cells Int 2022:3632549 (2022). PubMed: 35126526
- Yang G et al. The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling. Front Microbiol 13:911479 (2022). PubMed: 35770165