Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体(ab21536)
Key features and details
- Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: ELISA, WB, ICC
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体
参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗 -
描述
兔多克隆抗体to DDDDK tag (Binds to FLAG® tag sequence) -
宿主
Rabbit -
经测试应用
适用于: ELISA, WB, ICCmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
Sequence:XXXDDDDK
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常规说明
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯化说明
ECS (Enterokinase Cleavage Site) Affinity Purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21536于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
Use at a concentration of 2 - 10 µg/ml for coating or 0.03 - 1 µg/ml as a primary. |
|
| WB | (2) |
Use at an assay dependent concentration.
Use at a concentration of 0.1 - 1 µg/ml (colorimetric) or 0.03 - 1µg/ml (chemiluminescence). |
| ICC |
Use a concentration of 0.5 - 5 µg/ml.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. Use at a concentration of 2 - 10 µg/ml for coating or 0.03 - 1 µg/ml as a primary. |
|
WB
Use at an assay dependent concentration. Use at a concentration of 0.1 - 1 µg/ml (colorimetric) or 0.03 - 1µg/ml (chemiluminescence). |
|
ICC
Use a concentration of 0.5 - 5 µg/ml. |
靶标
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相关性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
别名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab21536 被引用在 24 文献中.
- Dichlberger A et al. LAPTM4B controls the sphingolipid and ether lipid signature of small extracellular vesicles. Biochim Biophys Acta Mol Cell Biol Lipids 1866:158855 (2021). PubMed: 33181324
- Rosales Gerpe MC et al. Optimized Pre-Clinical Grade Production of Two Novel Lentiviral Vector Pseudotypes for Lung Gene Delivery. Hum Gene Ther 31:459-471 (2020). PubMed: 32000531
- Lee YH et al. Chondroitin sulfate proteoglycan 4 regulates zebrafish body axis organization via Wnt/planar cell polarity pathway. PLoS One 15:e0230943 (2020). PubMed: 32240230
- Pradhan BS et al. Downregulation of Sostdc1 in Testicular Sertoli Cells is Prerequisite for Onset of Robust Spermatogenesis at Puberty. Sci Rep 9:11458 (2019). PubMed: 31391487
- Rosales Gerpe MC et al. The U3 and Env Proteins of Jaagsiekte Sheep Retrovirus and Enzootic Nasal Tumor Virus Both Contribute to Tissue Tropism. Viruses 11:N/A (2019). PubMed: 31739606