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Tags & Cell Markers Epitope Tags DDDDK Tag

Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体(ab1170)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (5)

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Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170)

    Key features and details

    • Chicken polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
    • Suitable for: ELISA, WB
    • Reacts with: Species independent
    • Isotype: IgY

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    概述

    • 产品名称

      Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体
      参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗
    • 描述

      鸡多克隆抗体to DDDDK tag (Binds to FLAG® tag sequence)
    • 宿主

      Chicken
    • 经测试应用

      适用于: ELISA, WBmore details
    • 种属反应性

      与反应: Species independent
    • 免疫原

      Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin. Peptide xxxDDDDK, xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.

    • 常规说明

      Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

      FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.20
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Antibodies were affinity purified using the peptide coupled to a different carrier.
    • 克隆

      多克隆
    • 同种型

      IgY
    • 研究领域

      • Tags & Cell Markers
      • Epitope Tags
      • DDDDK Tag

    相关产品

    • Isotype control

      • Chicken IgY - Isotype Control (ab50579)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1170于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ELISA
    1/5000 - 1/20000.
    WB (2)
    1/2000 - 1/10000.
    说明
    ELISA
    1/5000 - 1/20000.
    WB
    1/2000 - 1/10000.

    靶标

    • 相关性

      This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
    • 别名

      • DDDDK epitope tag antibody
      • DDDK antibody
      • ddk antibody
      • DYKDDDDK (also known as the FLAG® tag sequence) antibody
      • DYKDDDDK antibody
      • DYKDDDDK epitope tag antibody
      • DYKDDDDK tag antibody
      • ECS epitope tag antibody
      • ECS tag antibody
      • Enterokinase Cleavage Site epitope tag antibody
      • Enterokinase Cleavage Site tag antibody
      • FLAG® antibody
      • FLAG® tag antibody
      see all

    图片

    • Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170)
      Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170)This image is courtesy of an anonymous Abreview
      Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170) at 1/5000 dilution + Escherichia coli Recombinant protein (BL21)

      Secondary
      Rabbit Anti-Chicken IgY H&L (HRP) (ab6753) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 66 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds

      See Abreview

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (5)

    发表研究结果有使用 ab1170?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1170 被引用在 5 文献中.

    • Cao Y  et al. SIRT3 promotion reduces resistance to cisplatin in lung cancer by modulating the FOXO3/CDT1 axis. Cancer Med 10:1394-1404 (2021). PubMed: 33655712
    • Matsui H  et al. Cytosolic dsDNA of mitochondrial origin induces cytotoxicity and neurodegeneration in cellular and zebrafish models of Parkinson's disease. Nat Commun 12:3101 (2021). PubMed: 34035300
    • Menon A  et al. Electrophysiologic and molecular mechanisms of a frameshift NPPA mutation linked with familial atrial fibrillation. J Mol Cell Cardiol 132:24-35 (2019). PubMed: 31077706
    • Lim J  et al. Nuclear Proximity of Mtr4 to RNA Exosome Restricts DNA Mutational Asymmetry. Cell 169:523-537.e15 (2017). PubMed: 28431250
    • Hutson MR  et al. Temperature-activated ion channels in neural crest cells confer maternal fever-associated birth defects. Sci Signal 10:N/A (2017). PubMed: 29018170

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Primary hippocampal neurons)
    Permeabilization
    No
    Specification
    Primary hippocampal neurons
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 18 2013

    Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Recombinant protein (BL21)
    Gel Running Conditions
    Reduced Denaturing (4~20%)
    Loading amount
    0.1 µg
    Specification
    BL21
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 30 2010

    Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Purified protein (Mach1cell)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    0.5 µg
    Specification
    Mach1cell
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 09 2009

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