AB1162

Anti-DDDDK tag (Binds to FLAG® tag sequence) 抗体

Name: DDDDK tag (Binds to FLAG® tag sequence)抗体 (ab1162)| Abcam中文官网
Brand: Abcam Limited
SKU: AB1162
Price: 3959 CNY
Availability: InStock
Rating: 4 (15 reviews)

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

Lab Essentials
了解详情

(15 Reviews)

(208 Publications)

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) is a rabbit polyclonal antibody detecting DDDDK tag (Binds to FLAG® tag sequence) in Western Blot, ELISA.

- Over 160 publications
- Trusted since 2002

查看别名

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk

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Supplier Data

Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB1162)

Immunofluorescent analysis of A293 cells labeling FLAG^®-VPS35 with ab1162 at 10 μg/ml. The nuclear counter stain is DAPI (blue).

AbReview33620****

Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB1162)

ab1162 staining DDDDK tag in hTERT-RPE1 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Saponin and blocked with 0.02% BSA for 20 minutes at 18°C. Samples were incubated with primary antibody (1/100) for 45 minutes at 18°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

PubMed

Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB1162)

ab1162 staining DDDDK tag (pink) in neonatal rat cardiomyocytes transduced with Ad-TBX18/FLAG^®-IRES-GFP by Immunocytochemistry/ Immunofluorescence.

Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and incubated with primary antibody. An Alexa Fluor^®-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were stained with DAPI (blue).

Image from Kapoor N et al., J Biol Chem. 2011 Apr 22;286(16):14073-9. Epub 2011 Jan 4. Fig 1A.; doi: 10.1074/jbc.M110.185298; April 22, 2011 The Journal of Biological Chemistry, 286, 14073-14079.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

应用

WB, ICC, ELISA

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "As coating antibody in sELISA.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Antibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope\" R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope." } } }

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产品详情

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC, WB in human, mouse, rat samples.

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has been cited over 166 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has high sensitivity and specificity.

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has 13 independent reviews from customers.

DDDDK tag (Binds to FLAG® tag sequence) antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) has been validated in ELISA, ICC, WB.

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162) and is sold in 100 µg selling sizes.

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information DDDDK tag (Binds to FLAG® tag sequence)

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文献 (208)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw0730 PubMed40802746

2025

Lipid nanoparticles for mRNA delivery in brain via systemic administration.

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Species

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Dinglingge Cao,Xucheng Hou,Chang Wang,Siyu Wang,Zhengwei Liu,Meng Tian,Kaiyuan Guo,Haoyuan Li,Diana D Kang,Yichen Zhong,Yonger Xue,Changyue Yu,Binbin Deng,Yizhou Dong

iScience 28:113113 PubMed40792019

2025

NXF1 suppresses progression of endometrial cancer by interacting with the SRSF3 to regulate SP4 splicing.

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Species

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Nan Meng,Lingjun Li,Xueqin Yang,Hui Tang,Jizhong Wang,Songbai Zhang,Zongbao Xia,Jia Yao,Qi Zhang,Changrong Hu,Chunjie Su,Rui Duan

Nature communications 16:6923 PubMed40721560

2025

Inhibition of HDAC6 alters fumarate hydratase activity and mitochondrial structure.

Applications

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Species

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Andrew Roe,Catríona M Dowling,Cian D'Arcy,Daniel Alencar Rodrigues,Yu Wang,Matthew Hiller,Carl Keogh,Kate E R Hollinshead,Massimiliano Garre,Brenton Cavanagh,Kieran Wynne,Tianyan Liu,Zhixing Chen,Emma Kerr,Marie McIlroy,Jochen H M Prehn,Ingmar Schoen,Tríona Ní Chonghaile

Materials today. Bio 32:101878 PubMed40520558

2025

A pH-responsive PEG coating strategy for enhancing the enrichment of small extracellular vesicles towards disease regions with acidic microenvironment.

Applications

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Species

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Jianwei Zhao,Xinyu Niu,Lei Luo,Ji Yuan,Juntao Zhang,Xin Niu,Hengli Tian,Yunlong Yang,Zhifeng Deng,Yang Wang

International journal of medical sciences 22:1905-1915 PubMed40225867

2025

MUC15 acts as a tumor suppressor gene which correlates with prognosis and immune infiltration in esophageal squamous cell carcinoma.

Applications

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Species

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Xiang Fei,Ai-Li Wang,Hao Wu,Si-Wei Xing,Lei Chen,Ying Chen,Xue-Jing Lin,Hai-Long Liu,Bin Sun

The EMBO journal 44:54-74 PubMed39562740

2024

Intestinal NUCB2/nesfatin-1 regulates hepatic glucose production via the MC4R-cAMP-GLP-1 pathway.

Applications

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Species

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Shan Geng,Shan Yang,Xuejiao Tang,Shiyao Xue,Ke Li,Dongfang Liu,Chen Chen,Zhiming Zhu,Hongting Zheng,Yuanqiang Wang,Gangyi Yang,Ling Li,Mengliu Yang

Breast cancer research : BCR 26:155 PubMed39506852

2024

PTPN20 promotes metastasis through activating NF-κB signaling in triple-negative breast cancer.

Applications

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Species

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Xiaoxiao Zuo,Xiaohan Zhao,Xiaofei Zhang,Qingyuan Li,Xingyu Jiang,Shumei Huang,Xuwei Chen,Xiangfu Chen,Weihua Jia,Hequn Zou,Dongni Shi,Xueke Qian

Journal of cachexia, sarcopenia and muscle 15:2559-2573 PubMed39323088

2024

The Innovative Role of Nuclear Receptor Interaction Protein in Orchestrating Invadosome Formation for Myoblast Fusion.

Applications

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Species

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Hsin-Hsiung Chen,Chia-Yang Lin,Ya-Ju Han,Yun-Hsin Huang,Yi-Hsiang Liu,Wan-En Hsu,Li-Kai Tsai,Hsing-Jung Lai,Yeou-Ping Tsao,Hsiang-Po Huang,Show-Li Chen

International journal of molecular sciences 25: PubMed39201788

2024

Development of Fusion-Based Assay as a Drug Screening Platform for Nipah Virus Utilizing Baculovirus Expression Vector System.

Applications

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Species

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Indah Permata Sari,Christopher Llynard D Ortiz,Lee-Wei Yang,Ming-Hsiang Chen,Ming-Der Perng,Tzong-Yuan Wu

Journal of nanobiotechnology 22:486 PubMed39143545

2024

A structured biomimetic nanoparticle as inflammatory factor sponge and autophagy-regulatory agent against intervertebral disc degeneration and discogenic pain.

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Species

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Kanglu Li,Wenbo Yang,Xuanzuo Chen,Yihan Yu,Yiran Liu,Feifei Ni,Yan Xiao,Xiangcheng Qing,Sheng Liu,YuXin He,Baichuan Wang,Li Xu,Zengwu Shao,Lei Zhao,Yizhong Peng,Hui Lin

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