JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109027

Anti-DDB1 抗体 [EPR6089]

Anti-DDB1 antibody [EPR6089]

Be the first to review this product! Submit a review

|

(38 Publications)

Rabbit Recombinant Monoclonal DDB1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 38 publications.

查看别名

XAP1, DDB1, DNA damage-binding protein 1, DDB p127 subunit, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-damaged DNA-binding factor, UV-damaged DNA-binding protein 1, XPE-binding factor, Xeroderma pigmentosum group E-complementing protein, DDBa, XAP-1, UV-DDB 1, XPE-BF, XPCe

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody [EPR6089] (AB109027)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody [EPR6089] (AB109027)

Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109027 at a dilution of 1/100. Antigen retrieval was heat mediated before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (AB109027)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (AB109027)

ab109027 stained UV-treated HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109027 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (AB109027)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (AB109027)

Immunocytochemistry analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling DDB1 with ab109027 at 1/250 (8.9 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.

Confocal image showing nuclear and cytoplasmic staining in NIH/3T3 cells.

Western blot - Anti-DDB1 antibody [EPR6089] (AB109027)
  • WB

Unknown

Western blot - Anti-DDB1 antibody [EPR6089] (AB109027)

All lanes:

Western blot - Anti-DDB1 antibody [EPR6089] (ab109027) at 1/50000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

NIH3T3 cell lysate at 10 µg

Lane 4:

Human platelet lysate at 10 µg

Predicted band size: 127 kDa

false

OI-RD Scanning - Anti-DDB1 antibody [EPR6089] (AB109027)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-DDB1 antibody [EPR6089] (AB109027)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (9)

  • Carrier free

    Anti-DDB1 antibody [EPR6089] - BSA and Azide free

  • HRP

    HRP Anti-DDB1 antibody [EPR6089]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-DDB1 antibody [EPR6089]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-DDB1 antibody [EPR6089]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-DDB1 antibody [EPR6089]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-DDB1 antibody [EPR6089]

  • 578 PE

    PE Anti-DDB1 antibody [EPR6089]

  • 660 APC

    APC Anti-DDB1 antibody [EPR6089]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-DDB1 antibody [EPR6089]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6089

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Shipping conditions update: ambient shipping

This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.

Why the change?

It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.

What you need to know

  • Ambient shipments come clearly marked on the delivery note.
  • No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
  • Warranty coverage remains fully valid, aligned with our validated shipping method.
  • Please store the product as per the datasheet instructions upon receipt.

Find out more - https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
运输条件
Conditional Ambient
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DDB1 also known as Damage-Specific DNA Binding Protein 1 and DDB00A acts mechanically by recognizing and binding to DNA damage sites particularly UV-induced lesions. It functions as part of the UV-damaged DNA-binding protein complex. DDB1 has a molecular weight of approximately 127 kDa. It expresses in various tissues prominently in cells that are actively cycling. The protein often cooperates with other proteins to promote DNA repair and maintenance of genomic stability.
Biological function summary

In terms of cellular processes beyond DDB1's immediate interactions it plays a critical role in DNA repair mechanisms and the cell cycle. DDB1 is part of the larger CUL4-DDB1 ubiquitin ligase complex which targets specific proteins for ubiquitination and subsequent degradation therefore facilitating DNA repair and regulating cell cycle progression. This ability to target damaged proteins or signaling errors for removal helps maintain cellular health and preserve genetic information integrity.

Pathways

DDB1 is pivotal in the nucleotide excision repair pathway and the DNA damage response pathway. It interacts dynamically with proteins such as XPC (xeroderma pigmentosum group C) to initiate repair mechanisms ensuring the proper removal of damaged DNA sections. Moreover DDB1's role in ubiquitination connects it to pathways regulating protein turnover and cellular homeostasis interacting with the ubiquitin-proteasome system which is important for controlling protein degradation.

DDB1's malfunction or misregulation associates with conditions like xeroderma pigmentosum due to its role in DNA damage repair. It is also linked to certain cancer types where DNA repair deficiencies contribute to uncontrolled cell proliferation. Through these diseases DDB1 often interacts with proteins such as BRWD3 which may modulate DDB1's activity or stability influencing the disease outcomes by affecting the DNA repair capacity or recognizing specific proteins for degradation.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PubMed : 14739464, PubMed : 15448697, PubMed : 16260596, PubMed : 16407242, PubMed : 16407252, PubMed : 16482215, PubMed : 16940174, PubMed : 17079684). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (PubMed : 15448697, PubMed : 16260596, PubMed : 16407242, PubMed : 16940174). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (PubMed : 15448697, PubMed : 16260596, PubMed : 16407242, PubMed : 16940174). Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 14739464, PubMed : 16407252, PubMed : 16482215, PubMed : 17079684, PubMed : 18332868, PubMed : 18381890, PubMed : 19966799, PubMed : 22118460, PubMed : 25043012, PubMed : 25108355, PubMed : 28886238). The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1 (PubMed : 14739464, PubMed : 16407252, PubMed : 16482215, PubMed : 17079684, PubMed : 18332868, PubMed : 18381890, PubMed : 19966799, PubMed : 22118460, PubMed : 25043012, PubMed : 25108355). DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage (PubMed : 16473935, PubMed : 16678110, PubMed : 17041588, PubMed : 18593899). The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair (PubMed : 16473935, PubMed : 16678110, PubMed : 17041588, PubMed : 18593899). DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (PubMed : 15882621). DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication (PubMed : 17041588). DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR) (PubMed : 12732143). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed : 26431207). DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver (By similarity). By acting on TET dioxygenses, essential for oocyte maintenance at the primordial follicle stage, hence essential for female fertility (By similarity). Maternal factor required for proper zygotic genome activation and genome reprogramming (By similarity).
See full target information DDB1
style
left-column

文献 (38)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8051 PubMed40877279

2025

Translational repression by 4E-T is crucial to maintain the prophase-I arrest in vertebrate oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Heim,Shiya Cheng,Jan Orth,Florian Stengel,Melina Schuh,Thomas U Mayer

Nature communications 15:10044 PubMed39567493

2024

Two mechanisms repress cyclin B1 translation to maintain prophase arrest in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shiya Cheng,Melina Schuh

Journal of translational medicine 22:834 PubMed39261935

2024

USP14 inhibition promotes DNA damage repair and represses ovarian granulosa cell senescence in premature ovarian insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Zi Ma,Ao Wang,Yun-Hui Lai,Jun Zhang,Xiao-Fei Zhang,Shi-Ling Chen,Xing-Yu Zhou

Development (Cambridge, England) 151: PubMed38785133

2024

Multiple intersecting pathways are involved in CPEB1 phosphorylation and regulation of translation during mouse oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chisato Kunitomi,Mayra Romero,Enrico Maria Daldello,Karen Schindler,Marco Conti

Nucleic acids research 52:1258-1271 PubMed38048302

2023

Alternative cleavage and polyadenylation of the Ccnb1 mRNA defines accumulation of cyclin protein during the meiotic cell cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotian Wang,Fang-Shiuan Leung,Jeffrey O Bush,Marco Conti

Nature chemical biology 19:1513-1523 PubMed37653169

2023

Activity-based profiling of cullin-RING E3 networks by conformation-specific probes.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas T Henneberg,Jaspal Singh,David M Duda,Kheewoong Baek,David Yanishevski,Peter J Murray,Matthias Mann,Sachdev S Sidhu,Brenda A Schulman

BMC cancer 23:399 PubMed37142958

2023

PTEN-negative endometrial cancer cells protect their genome through enhanced DDB2 expression associated with augmented nucleotide excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Fathima Hameed J S,Anjali Devarajan,Devu Priya M S,Ahel Bhattacharyya,Mayur Balkrishna Shirude,Debasree Dutta,Parimal Karmakar,Ananda Mukherjee

EMBO molecular medicine 15:e15924 PubMed36947051

2023

Targeting WDxR motif reprograms immune microenvironment and inhibits hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhang,Gang Chen,Xing Feng,Huiwen Song,Lingbing Meng,Yao Fu,Jun Yang,Zhiwen Fan,Youxiang Ding,Zhijie Du,Jianchao Wang,Li Yang,Jun Zhang,Lixia Sun,Zhigang Liu,Zhiyong Zhang,Quanhai Li,Xiangshan Fan

BMC biology 20:294 PubMed36575438

2022

SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Rongfang Qiu,Siyu Zhao,Lin Shen,Bufu Tang,Qiaoyou Weng,Ziwei Xu,Liyun Zheng,Weiqian Chen,Gaofeng Shu,Yajie Wang,Zhongwei Zhao,Minjiang Chen,Jiansong Ji

Nature cardiovascular research 1:1195-1214 PubMed39196168

2022

Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Liyong Zhang,David Smyth,Mohammad Al-Khalaf,Alice Blet,Qiujiang Du,Jordan Bernick,Michael Gong,Xu Chi,Yena Oh,Malaika Roba-Oshin,Elizabeth Coletta,Michel Feletou,Anthony O Gramolini,Kyoung-Han Kim,Thais Coutinho,James L Januzzi,Benoit Tyl,Andre Ziegler,Peter P Liu
View all publications
style
left-column
style
left-column
style
right-column

替代产品

Primary Antibodies

AB97522

Anti-DDB1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (AB97522)

  • 4
  • 4
Primary Antibodies

AB9194

Anti-DDB1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (AB9194)

  • 4
  • 2
Primary Antibodies

AB124672

Anti-DDB1 antibody [EPR6088]

RabMAb|Recombinant|Trial Size

Western blot - Anti-DDB1 antibody [EPR6088] (AB124672)

  • 3
  • 1

搭配使用产品

Primary Antibodies

AB181136

Anti-DDB2 antibody [EPR9811]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB2 antibody [EPR9811] (AB181136)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB92554

Anti-Cullin 4A/CUL-4A antibody [EPR3198]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (AB92554)

  • 3
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com