Anti-DDB1抗体(ab97522)
Key features and details
- Rabbit polyclonal to DDB1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-DDB1抗体
参阅全部 DDB1 一抗 -
描述
兔多克隆抗体to DDB1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow
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免疫原
Recombinant fragment corresponding to Human DDB1 aa 749-1123.
Database link: Q16531 -
阳性对照
- WB: NIH/3T3, PC-12, Rat2, HEK-293T, A431, HeLa and HepG2 whole cell lysate. IHC-P: Cal27 Xenograft, rat fore brain tissue. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97522于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/500 - 1/10000. Predicted molecular weight: 127 kDa.
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| IHC-P |
1/100 - 1/1000.
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| ICC/IF | (1) |
1/100 - 1/1000.
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| 说明 |
|---|
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WB
1/500 - 1/10000. Predicted molecular weight: 127 kDa. |
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IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
靶标
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功能
Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the DDB1 family. -
翻译后修饰
Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. -
细胞定位
Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 511951 Cow
- Entrez Gene: 100290337 Human
- Entrez Gene: 1642 Human
- Entrez Gene: 13194 Mouse
- Entrez Gene: 64470 Rat
- Omim: 600045 Human
- SwissProt: A1A4K3 Cow
- SwissProt: Q16531 Human
see all -
别名
- Damage specific DNA binding protein 1 antibody
- Damage-specific DNA-binding protein 1 antibody
- DDB 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)
Immunohistochemical analysis of paraffin-embedded Human Cal27 Xenograft , using ab97522 at 1/500 dilution.
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Western blot - Anti-DDB1 antibody (ab97522)All lanes : Anti-DDB1 antibody (ab97522) at 1/1000 dilution
Lane 1 : PC-12 whole cell extracts
Lane 2 : Rat2 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 127 kDa5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody (ab97522)ab97522 at 1/200 dilution staining DDB1 in Paraformaldehyde fixed HeLa cells. Lower image is merged with DNA probe. -
Western blot - Anti-DDB1 antibody (ab97522)Anti-DDB1 antibody (ab97522) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 127 kDa5% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)Paraffin embedded mouse prostate tissue stained for DDB1 using ab97522 at 1/500 dilutions in immunohistochemical analysis.
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Western blot - Anti-DDB1 antibody (ab97522)Anti-DDB1 antibody (ab97522) at 1/1000 dilution + NIH/3T3 whole cell lysate at 30 µg
Predicted band size: 127 kDa
7.5% SDS-PAGE. -
Western blot - Anti-DDB1 antibody (ab97522)All lanes : Anti-DDB1 antibody (ab97522) at 1/1000 dilution
Lane 1 : HEK-293T whole cell extract
Lane 2 : A431 whole cell extract
Lane 3 : HeLa whole cell extract
Lane 4 : HepG2 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 127 kDa5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab97522)Paraffin embedded rat fore brain tissue stained for DDB1 using ab97522 at 1/500 dilutions in immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab97522 被引用在 5 文献中.
- Marchetti AL et al. Proteomic Analysis of Nuclear Hepatitis B Virus Relaxed Circular DNA-Associated Proteins Identifies UV-Damaged DNA Binding Protein as a Host Factor Involved in Covalently Closed Circular DNA Formation. J Virol 96:e0136021 (2022). PubMed: 34705558
- Kusama K et al. Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers. Life (Basel) 12:N/A (2022). PubMed: 35207596
- Tateno S et al. Genome-wide screening reveals a role for subcellular localization of CRBN in the anti-myeloma activity of pomalidomide. Sci Rep 10:4012 (2020). PubMed: 32132601
- Zhu B et al. The protective role of DOT1L in UV-induced melanomagenesis. Nat Commun 9:259 (2018). PubMed: 29343685
- Parsons JL et al. Phosphorylation of PNKP by ATM prevents its proteasomal degradation and enhances resistance to oxidative stress. Nucleic Acids Res : (2012). WB ; Human . PubMed: 23042680