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Neuroscience Neurotransmission Nitric Oxide NOS

Anti-DDAH1抗体(ab2231)

  • Datasheet
Reviews (3)Q&A (1)References (15)

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Flow Cytometry - Anti-DDAH1 antibody (ab2231)
  • Immunocytochemistry - Anti-DDAH1 antibody (ab2231)
  • Immunocytochemistry - Anti-DDAH1 antibody (ab2231)
  • Western blot - Anti-DDAH1 antibody (ab2231)
  • Western blot - Anti-DDAH1 antibody (ab2231)

Key features and details

  • Goat polyclonal to DDAH1
  • Suitable for: ICC, Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-DDAH1抗体
    参阅全部 DDAH1 一抗
  • 描述

    山羊多克隆抗体to DDAH1
  • 宿主

    Goat
  • 经测试应用

    适用于: ICC, Flow Cyt, WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide corresponding to Human DDAH1 aa 273-285 (C terminal).
    Sequence:

    TCCSVLINKKVDS


    Database link: O94760
    (Peptide available as ab99047)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: HepG2 and LnCaP cell lysates. Human cerebellum, kidney and testes. ICC: HeLa and U20S cells Flow cyt: HeLa cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human DDAH1 protein (ab99863)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2231于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use a concentration of 10 µg/ml.
Flow Cyt
Use a concentration of 10 µg/ml.
WB (3)
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

1 hour primary incubation is recommended for this product.

说明
ICC
Use a concentration of 10 µg/ml.
Flow Cyt
Use a concentration of 10 µg/ml.
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

1 hour primary incubation is recommended for this product.

靶标

  • 功能

    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • 组织特异性

    Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
  • 序列相似性

    Belongs to the DDAH family.
  • Target information above from: UniProt accession O94760 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 23576 Human
    • Omim: 604743 Human
    • SwissProt: P56965 Cow
    • SwissProt: O94760 Human
    • Unigene: 379858 Human
    • Unigene: 713411 Human
    • 别名

      • DDAH antibody
      • DDAH I antibody
      • DDAH-1 antibody
      • DDAH1 antibody
      • DDAH1_HUMAN antibody
      • DDAHI antibody
      • Dimethylargininase 1 antibody
      • Dimethylargininase-1 antibody
      • Dimethylarginine dimethylaminohydrolase 1 antibody
      • N(G) antibody
      • N(G)-dimethylarginine dimethylaminohydrolase 1 antibody
      • NG NG dimethylarginine dimethylaminohydrolase antibody
      see all

    图片

    • Flow Cytometry - Anti-DDAH1 antibody (ab2231)
      Flow Cytometry - Anti-DDAH1 antibody (ab2231)
      Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton using ab2231 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 1ug/ml. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

       

    • Immunocytochemistry - Anti-DDAH1 antibody (ab2231)
      Immunocytochemistry - Anti-DDAH1 antibody (ab2231)
      Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton using ab2231 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 2ug/ml, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody.

       

    • Immunocytochemistry - Anti-DDAH1 antibody (ab2231)
      Immunocytochemistry - Anti-DDAH1 antibody (ab2231)
      Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton using ab2231 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 2ug/ml, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody.
    • Western blot - Anti-DDAH1 antibody (ab2231)
      Western blot - Anti-DDAH1 antibody (ab2231)
      All lanes : Anti-DDAH1 antibody (ab2231) at 0.3 µg/ml

      Lane 1 : Human cerebellum
      Lane 2 : Human kidney
      Lane 3 : Human testes

      Predicted band size: 31 kDa

    • Western blot - Anti-DDAH1 antibody (ab2231)
      Western blot - Anti-DDAH1 antibody (ab2231)
      All lanes : Anti-DDAH1 antibody (ab2231) at 0.3 µg/ml

      Lane 1 : HepG2 cell lysate
      Lane 2 : LnCaP cell lysate

      Predicted band size: 31 kDa

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (15)

    发表研究结果有使用 ab2231?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2231 被引用在 15 文献中.

    • Ding X  et al. Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer. Cancer Manag Res 13:1-11 (2021). PubMed: 33442289
    • Zhu ZD  et al. DDAH2 alleviates myocardial fibrosis in diabetic cardiomyopathy through activation of the DDAH/ADMA/NOS/NO pathway in rats. Int J Mol Med 43:749-760 (2019). PubMed: 30569164
    • Joris V  et al. MicroRNA-199a-3p and MicroRNA-199a-5p Take Part to a Redundant Network of Regulation of the NOS (NO Synthase)/NO Pathway in the Endothelium. Arterioscler Thromb Vasc Biol 38:2345-2357 (2018). PubMed: 29976767
    • Balasubramanian V  et al. Post-Transcriptional Regulation of Hepatic DDAH1 with TNF Blockade Leads to Improved eNOS Function and Reduced Portal Pressure In Cirrhotic Rats. Sci Rep 7:17900 (2017). WB ; Rat . PubMed: 29263339
    • Hulin JA  et al. MiR-193b regulates breast cancer cell migration and vasculogenic mimicry by targeting dimethylarginine dimethylaminohydrolase 1. Sci Rep 7:13996 (2017). PubMed: 29070803
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-DDAH1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain (Hippocampus))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Brain (Hippocampus)
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    圭一 角山

    Verified customer

    提交于 Nov 25 2015

    Western blot abreview for Anti-DDAH1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (hippocampus)
    Loading amount
    5 µg
    Specification
    hippocampus
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 09 2010

    Western blot abreview for Anti-DDAH1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Hippocampus lysate)
    Loading amount
    40 µg
    Specification
    Hippocampus lysate
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Richard D'Mello

    Verified customer

    提交于 Apr 14 2009

    Question

    Hi! So, we had a customer call today who is using ab 2231 in both human and rat aortic homogenate. He has loaded from 100 ugs to 1 mg of protein per lane diluted the antibody 5 ugs/ml and incubated overnight. He sees a band at 32kDa but nothing at 40 kDa. It says on the datasheet that the predicted MW is 33 kDa but runs at 40 kDa do you have the peptide available for them to run a blocking study? The other issue is that they see this band in their negative control lane (with BSA). Otherwise, this looks to be an issue with the antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 20 2003

    Answer

    According to the datasheet, this antibody is thought to run at 40kDa due glycosylation. It could be that the protein is running at 32kDa in their experiments as the protein in their system is not glycosylated. However, I am concerned that their negative control is also showing a band at the same size. We do not have the peptide here for them to try but I will ask the originator of the antibody if they have the blocking peptide available. It would help if the researcher could contact us directly and provide a blot image. Are they just loading BSA in their negative control lane? Please ask them to contact us by e-mailing technical@abcam.com and attaching a blot image to the e-mail.

    Read More

    Abcam Scientific Support

    回复于 Jun 20 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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