Anti-DCLK2抗体(ab106639)
Key features and details
- Rabbit polyclonal to DCLK2
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-DCLK2抗体 -
描述
兔多克隆抗体to DCLK2 -
宿主
Rabbit -
特异性
Based on analysis of the immunogen sequence, we would expect this antibody to react with all three described isoforms of human DCLK2 (Q8N568-1, -2,-3) isoforms 1 and 3 of rat DCLK2 (Q5MPA9-1,-3) but not isoform 2 (Q5MPA9-2), and isoforms 1, 2, and 3 (Q6PGN3-1,-2,-3) of mouse DCLK2 but not isoform 4, 5, and 6 (Q6PGN3-4,-5,-6).
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经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human DCLK2 (C terminal).
Database link: NP_001035350 -
阳性对照
- Rat brain tissue and tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
ab106639 is purified by affinity chromatography via a peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106639于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 84 kDa.
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| 说明 |
|---|
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IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 84 kDa. |
靶标
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功能
Protein kinase with a significantly reduced Ca2+/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 2 doublecortin domains.
Contains 1 protein kinase domain. -
结构域
The doublecortin domains are involved in the colocalization with microtubules. -
细胞定位
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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数据库链接
- Entrez Gene: 70762 Mouse
- Entrez Gene: 310698 Rat
- SwissProt: Q6PGN3 Mouse
- SwissProt: Q5MPA9 Rat
- Unigene: 44490 Mouse
- Unigene: 23327 Rat
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别名
- CaMK-like CREB regulatory kinase 2 antibody
- CL2 antibody
- CLICK-II antibody
see all
图片
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Western blot - Anti-DCLK2 antibody (ab106639)
Western Blot of rat brain tissue lysate labeling DCLK2 with Anti-DCLK2 antibody (ab106639) at (1) 1 and (2) 2μg/ml.
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Western blot - Anti-DCLK2 antibody (ab106639)Lane 1 : Anti-DCLK2 antibody (ab106639) at 1 µg/ml
Lane 2 : Anti-DCLK2 antibody (ab106639) at 2 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 84 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCLK2 antibody (ab106639)ab106639 at 5µg/ml, staining DCLK2 in Rat brain, using Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab106639 被引用在 3 文献中.
- DeOliveira-Mello L et al. Doublecortin in the Fish Visual System, a Specific Protein of Maturing Neurons. Biology (Basel) 11:N/A (2022). PubMed: 35205114
- Vilallongue N et al. Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system. Nat Commun 13:6040 (2022). PubMed: 36229455
- Lipka J et al. Microtubule-binding protein doublecortin-like kinase 1 (DCLK1) guides kinesin-3-mediated cargo transport to dendrites. EMBO J 35:302-18 (2016). PubMed: 26758546