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重组RabMAb

重组Anti-DCK抗体[EPR29105-53] - BSA and Azide free (ab317830)

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Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Immunocytochemistry/ Immunofluorescence - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Flow Cytometry (Intracellular) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Immunoprecipitation - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Immunoprecipitation - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
  • Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR29105-53] to DCK - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-DCK抗体[EPR29105-53] - BSA and Azide free
    参阅全部 DCK 一抗

  • 描述

    兔单克隆抗体[EPR29105-53] to DCK - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    Unsuitable for human ICC and mouse FC-intra.

  • 经测试应用

    适用于: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Mouse, Human
    不与反应: Rat

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: 293T, HCT 116, HeLa, IMR-90, RAW 264.7, A20, Neuro-2a, Human spleen, Human thymus, Mouse thymus and Mouse spleen lysates. IHC-P: Human tonsil, Mouse spleen, Mouse thymus, Mouse T- lymphoma and Mouse cardiac muscle tissues. ICC/IF: RAW 264.7 cells. Flow Cyt (Intra): HeLa cell. IP: HeLa and RAW 264.7 cells.

  • 常规说明

    ab317830 is the carrier-free version of ab317829

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317830于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

靶标

  • 功能

    Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.

  • 序列相似性

    Belongs to the DCK/DGK family.

  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • dCK antibody
      • DCK protein antibody
      • DCK_HUMAN antibody
      • Deoxycytidine kinase antibody
      • Deoxynucleoside kinase antibody
      • EC 2.7.1 antibody
      • EC 2.7.1.74 antibody
      • MGC117410 antibody
      • MGC138632 antibody
      • OTTHUMP00000219118 antibody
      • OTTHUMP00000219119 antibody
      see all

    图片

    • Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      All lanes : Anti-DCK antibody [EPR29105-53] (ab317829) at 1/1000 dilution

      Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
      Lane 2 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
      Lane 3 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : IMR-90 (human lung fibroblast) whole cell lysate
      Lane 5 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 6 : A20 (mouse reticulum sarcoma b lymphocyte) whole cell lysate
      Lane 7 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 31 kDa


      Exposure time: 180 seconds


      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: IMR-90 (PMID: 33556172), A20.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      All lanes : Anti-DCK antibody [EPR29105-53] (ab317829) at 1/1000 dilution

      Lane 1 : Human spleen tissue lysate
      Lane 2 : Human placenta tissue lysate
      Lane 3 : Human thymus tissue lysate
      Lane 4 : Human liver tissue lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 31 kDa
      Observed band size: 31 kDa


      Exposure time: 180 seconds


      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: liver (PMID: 7929097, PMID: 9079672), placenta (PMID: 9079672).

      The identity of the bands higher than 25 kDa (in lane 2) are unknown.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      All lanes : Anti-DCK antibody [EPR29105-53] (ab317829) at 1/1000 dilution

      Lane 1 : Mouse thymus tissue lysate
      Lane 2 : Mouse liver tissue lysate
      Lane 3 : Mouse heart tissue lysate
      Lane 4 : Mouse spleen tissue lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 31 kDa


      Exposure time: 59 seconds


      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: liver, heart (PMID: 7929097).

      The identity of the higher MW band at approximately 40 kDa (in lane 2) is unknown.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human tonsil (PMID: 29550884). The section was incubated with ab317829 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on mouse spleen. The section was incubated with ab317829 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on mouse thymus. The section was incubated with ab317829 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse T-cell lymphoma tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on mouse T-cell lymphoma. The section was incubated with ab317829 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression: no staining on human cardiac muscle. The section was incubated with ab317829 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression: scattered positive staining on mouse cardiac muscle. The section was incubated with ab317829 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunocytochemistry/ Immunofluorescence - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Immunocytochemistry/ Immunofluorescence - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

      Confocal image showing nuclear staining in RAW 264.7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
      Low expression: A20.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    • Flow Cytometry (Intracellular) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Flow Cytometry (Intracellular) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) (Right) / IMR-90 (human lung fibroblast) (Left) cells labelling DCK with ab317829 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Low expression: IMR-90 (PMID: 33556172).

    • Immunoprecipitation - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Immunoprecipitation - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      DCK was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317829 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317829 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
      Lane 2: ab317829 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317829 in HeLa whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 180 seconds..

    • Immunoprecipitation - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Immunoprecipitation - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

      This data was developed using ab317829, the same antibody clone in a different buffer formulation.

      DCK was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab317829 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317829 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 2: ab317829 IP in RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317829 in RAW 264.7 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 180 seconds..

    • Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)
      Anti-DCK antibody [EPR29105-53] - BSA and Azide free (ab317830)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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    ab317830 尚未被引用在任何文献中。

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