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AB317830

重组Anti-DCK抗体[EPR29105-53] - BSA and Azide free

Anti-DCK antibody [EPR29105-53] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal DCK antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P, IP, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.

查看别名

Deoxycytidine kinase, dCK, Deoxyadenosine kinase, Deoxyguanosine kinase, DCK

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : no staining on human cardiac muscle. The section was incubated with ab317829 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil (PMID : 29550884). The section was incubated with ab317829 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) (Right) / IMR-90 (human lung fibroblast) (Left) cells labelling DCK with ab317829 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : IMR-90 (PMID : 33556172).

Immunoprecipitation - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • IP

Supplier Data

Immunoprecipitation - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

DCK was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317829 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317829 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab317829 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317829 in HeLa whole cell lysate

All lanes:

Immunoprecipitation - Anti-DCK antibody [EPR29105-53] (<a href='/products/primary-antibodies/dck-antibody-epr29105-53-ab317829'>ab317829</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse thymus. The section was incubated with ab317829 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : scattered positive staining on mouse cardiac muscle. The section was incubated with ab317829 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse T-cell lymphoma tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse T-cell lymphoma. The section was incubated with ab317829 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse spleen. The section was incubated with ab317829 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling DCK with ab317829 at 1/500 (1.008 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing nuclear staining in RAW 264.7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Low expression : A20.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunoprecipitation - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • IP

Supplier Data

Immunoprecipitation - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

DCK was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab317829 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317829 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : ab317829 IP in RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317829 in RAW 264.7 whole cell lysate

All lanes:

Immunoprecipitation - Anti-DCK antibody [EPR29105-53] (<a href='/products/primary-antibodies/dck-antibody-epr29105-53-ab317829'>ab317829</a>) at 1/30 dilution

All lanes:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • WB

Supplier Data

Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Low expression : IMR-90 (PMID : 33556172), A20.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-DCK antibody [EPR29105-53] (<a href='/products/primary-antibodies/dck-antibody-epr29105-53-ab317829'>ab317829</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 30 µg

Lane 2:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 30 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 4:

IMR-90 (human lung fibroblast) whole cell lysate at 30 µg

Lane 5:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 30 µg

Lane 6:

A20 (mouse reticulum sarcoma b lymphocyte) whole cell lysate at 30 µg

Lane 7:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 31 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • WB

Supplier Data

Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Low expression : liver (PMID : 7929097, PMID : 9079672), placenta (PMID : 9079672).

The identity of the bands higher than 25 kDa (in lane 2) are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DCK antibody [EPR29105-53] (<a href='/products/primary-antibodies/dck-antibody-epr29105-53-ab317829'>ab317829</a>) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 30 µg

Lane 2:

Human placenta tissue lysate at 30 µg

Lane 3:

Human thymus tissue lysate at 30 µg

Lane 4:

Human liver tissue lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 31 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)
  • WB

Supplier Data

Western blot - Anti-DCK antibody [EPR29105-53] - BSA and Azide free (AB317830)

This data was developed using ab317829, the same antibody clone in a different buffer formulation.

Low expression : liver, heart (PMID : 7929097).

The identity of the higher MW band at approximately 40 kDa (in lane 2) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DCK antibody [EPR29105-53] (<a href='/products/primary-antibodies/dck-antibody-epr29105-53-ab317829'>ab317829</a>) at 1/1000 dilution

Lane 1:

Mouse thymus tissue lysate at 30 µg

Lane 2:

Mouse liver tissue lysate at 30 µg

Lane 3:

Mouse heart tissue lysate at 30 µg

Lane 4:

Mouse spleen tissue lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 31 kDa,36 kDa

false

Exposure time: 59s

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR29105-53

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse

应用

Flow Cyt (Intra), IHC-P, IP, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Unsuitable for human ICC and mouse FC-intra.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

ab317830 is the carrier-free version of ab317829.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Phosphorylates the deoxyribonucleosides deoxycytidine, deoxyguanosine and deoxyadenosine (PubMed : 12808445, PubMed : 18377927, PubMed : 19159229, PubMed : 1996353, PubMed : 20614893, PubMed : 20637175). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents (PubMed : 12808445).
See full target information DCK
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Society of Nephrology : JASN 27:3600-3610 PubMed27026370

2016

APOL1-G0 or APOL1-G2 Transgenic Models Develop Preeclampsia but Not Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Leslie A Bruggeman,Zhenzhen Wu,Liping Luo,Sethu M Madhavan,Martha Konieczkowski,Paul E Drawz,David B Thomas,Laura Barisoni,John R Sedor,John F O'Toole
View all publications
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搭配使用产品

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR6114] (AB133582)

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Abcam Product Promise

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详情请参阅我们的条款与条件。

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