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AB322504

Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector)

Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal DCD antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

AIDD, DSEP, DCD, Dermcidin, Preproteolysin

3 Images
Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)

Sandwich ELISA of ab322513 using human DCD immunogen protein standard curve with the capture antibody ab322503 at 2 μg/mL and detector antibody ab322504 at 0.5 μg/mL. Background-subtracted absorbance data values (mean +/- standard deviation) are displayed.

Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)

Interpolated concentration of DCD was measured in duplicate at different sample concentrations. Undiluted sample is a 1 : 4 dilution from neat serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DCD antibody [EPR25718-6] - BSA and Azide free (Detector) (AB322504)

Interpolated concentration of DCD was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Skin tissue extract 3.9 µg/ml and cerebellum tissue extract 3.9 µg/ml. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). In the cerebellum tissue extract the signal was not detectable (ND). Data represented here are indicative of assay working using example of biological samples. However, we do not guarantee same sensitivity level due to biological variation.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25718-6

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab322504 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human DCD. The recommended pair for sandwich ELISA is:
Capture: ab322503, Human DCD Capture Antibody (unconjugated)
Detector: ab322504, Human DCD Detector Antibody (unconjugated)
The reference range value is 4.8-156 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

DCD-1. Found in sweat, has an antimicrobial activity during early bacterial colonization (PubMed : 11694882, PubMed : 23426625). The secreted peptide assembles into homohexameric complexes that can associate with and also insert into pathogen membranes (PubMed : 23426625). Once inserted in bacteria membranes forms anion channels probably altering the transmembrane potential essential for bacterial survival (PubMed : 23426625). Highly effective against E.coli, E.faecalis, S.aureus and C.albicans (PubMed : 11694882). Optimal pH and salt concentration resemble the conditions in sweat (PubMed : 11694882). Also exhibits proteolytic activity, cleaving on the C-terminal side of Arg and, to a lesser extent, Lys residues (PubMed : 17448443).. Survival-promoting peptide. Promotes survival of neurons and displays phosphatase activity (PubMed : 9736629). It may bind IgG (PubMed : 9736629).
See full target information DCD
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Abcam Product Promise

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