DCC重组抗体[EPR23313-115]_DCC抗体(ab273570)| Abcam中文官网

产品名称

Anti-DCC抗体[EPR23313-115]
参阅全部 DCC 一抗
描述

兔单克隆抗体[EPR23313-115] to DCC
宿主

Rabbit
经测试应用

适用于: Flow Cyt, IHC-Fr, IHC-P, IP, WBmore details
不适用于: ICC/IF
种属反应性

与反应: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Human hippocampus tissue lysate; Mouse P5 hippocampus tissue lysate; Rat P7 hippocampus and Rat E18 brain tissue lysates. IHC-P: Human cerebrum and Mouse E14.5, Rat E14.5 tissues. IHC-Fr: Mouse E14.5 embryo and Rat E14.5 embryo tissue. Flow Cyt: Rat primary neuron cells. IP: Human hippocampus tissue lysate; Mouse P5 cerebral cortex tissue lysate.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR23313-115
同种型

IgG
研究领域
- Neuroscience
- Development

Alternative Versions
- Anti-DCC antibody [EPR23313-115] - BSA and Azide free (ab273576)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

The Abpromise guarantee

Abpromise™承诺保证使用ab273570于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt		1/50.
IHC-Fr		1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		1/30.
WB		1/1000. Detects a band of approximately 175, 240 kDa (predicted molecular weight: 158 kDa).

说明
Flow Cyt 1/50.
IHC-Fr 1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.
WB 1/1000. Detects a band of approximately 175, 240 kDa (predicted molecular weight: 158 kDa).

应用说明

Is unsuitable for ICC/IF.

功能

Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.
组织特异性

Found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Not expressed in colorectal tumor cells that lost their capacity to differentiate into mucus producing cells.
疾病相关

Defects in DCC are the cause of congenital mirror movements (MIMOC) [MIM:157600]. Mirror movements are contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities.
序列相似性

Belongs to the immunoglobulin superfamily. DCC family.
Contains 6 fibronectin type-III domains.
Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
翻译后修饰

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
细胞定位

Membrane.
Target information above from: UniProt accession P43146 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 1630 Human
- Entrez Gene: 13176 Mouse
- Entrez Gene: 25311 Rat
- Omim: 120470 Human
- SwissProt: P43146 Human
- SwissProt: P70211 Mouse
- SwissProt: Q63155 Rat
- Unigene: 162025 Human
- Unigene: 167882 Mouse
see all
别名
- Colorectal cancer related chromosome sequence 18 antibody
- Colorectal cancer suppressor antibody
- CRC 18 antibody
- CRC18 antibody
- CRCR 1 antibody
- CRCR1 antibody
- DCC antibody
- DCC_HUMAN antibody
- Deleted in colorectal cancer protein antibody
- Deleted in colorectal carcinoma antibody
- Immunoglobulin superfamily DCC subclass member 1 antibody
- Netrin receptor DCC antibody
- Tumor suppressor protein DCC antibody
see all

Western blot - Anti-DCC antibody [EPR23313-115] (ab273570)

All lanes : Anti-DCC antibody [EPR23313-115] (ab273570) at 1/1000 dilution

Lane 1 : Human hippocampus tissue lysate
Lane 2 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Mouse P5 hippocampus tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

Predicted band size: 158 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

DCC is a glycoprotein. The molecular weight observed is consistent with what have been described in the literature (PMID: 8044801).

Negative control: SW480 (PMID: 7926722).

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCC antibody [EPR23313-115] (ab273570)

Immunohistochemical analysis of paraffin-embedded Mouse E14.5 tissue labeling DCC with ab273570 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse E14.5 cerebrum (PMID: 24739528). The section was incubated with ab273570 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-DCC antibody [EPR23313-115] (ab273570)

All lanes : Anti-DCC antibody [EPR23313-115] (ab273570) at 1/1000 dilution

Lane 1 : Rat P7 hippocampus tissue lysate
Lane 2 : Rat E18 brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 158 kDa
Observed band size: 175,240 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

DCC is a glycoprotein. The molecular weight observed is consistent with what have been described in the literature (PMID: 8044801).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time: 26 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCC antibody [EPR23313-115] (ab273570)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling DCC with ab273570 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum (PMID: 11684721). The section was incubated with ab273570 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-DCC antibody [EPR23313-115] (ab273570)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat E14.5 embryo tissue labeling DCC with ab273570 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat embryonic brain is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunoprecipitation - Anti-DCC antibody [EPR23313-115] (ab273570)

DCC was immunoprecipitated from 0.35 mg Mouse P5 cerebral cortex tissue lysate with ab273570 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273570 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse P5 cerebral cortex tissue lysate 10 ug

Lane 2: ab273570 IP in Mouse P5 cerebral cortex tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273570 in Mouse P5 cerebral cortex tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 32 seconds.
Flow Cytometry - Anti-DCC antibody [EPR23313-115] (ab273570)

Flow cytometric analysis of PC-12 (Rat adrenal gland pheochromocytoma, Left) / Rat primary neuron cells (Right) cells labelling DCC with ab273570 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control: PC12 (PMID: 8044801).

Gated on viable cells.
Immunohistochemistry (Frozen sections) - Anti-DCC antibody [EPR23313-115] (ab273570)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryo tissue labeling DCC with ab273570 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse embryonic brain is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunoprecipitation - Anti-DCC antibody [EPR23313-115] (ab273570)

DCC was immunoprecipitated from 0.35 mg Human hippocampus tissue lysate with ab273570 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273570 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human hippocampus tissue lysate 10 ug

Lane 2: ab273570 IP in Human hippocampus tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273570 in human hippocampus tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Flow Cytometry - Anti-DCC antibody [EPR23313-115] (ab273570)

Flow cytometric analysis of Rat primary neuron cells cells labelling DCC with ab273570 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCC antibody [EPR23313-115] (ab273570)

Immunohistochemical analysis of paraffin-embedded Rat E14.5 tissue labeling DCC with ab273570 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat E14.5 cerebrum (PMID: 24739528). The section was incubated with ab273570 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-DCC antibody [EPR23313-115] (ab273570)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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发表研究结果有使用 ab273570？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab273570 被引用在 3 文献中.

Li Y et al. Gαi1/3 mediate Netrin-1-CD146-activated signaling and angiogenesis. Theranostics 13:2319-2336 (2023). PubMed: 37153740
Taylor HA et al. PTPRD and DCC Are Novel BACE1 Substrates Differentially Expressed in Alzheimer's Disease: A Data Mining and Bioinformatics Study. Int J Mol Sci 23:N/A (2022). PubMed: 35562959
Wang J et al. Upregulation of Netrin-1 in the hippocampus mediates the formation of visceral hypersensitivity induced by maternal separation. Front Mol Neurosci 15:908911 (2022). PubMed: 35966013

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概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (3)

客户评价及客户问答