Anti-DC-SIGN抗体(ab5716)
Key features and details
- Rabbit polyclonal to DC-SIGN
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DC-SIGN抗体
参阅全部 DC-SIGN 一抗 -
描述
兔多克隆抗体to DC-SIGN -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human DC-SIGN aa 277-293.
(Peptide available asab6092) -
阳性对照
- Human placenta tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
靶标
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功能
Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells. -
组织特异性
Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. -
序列相似性
Contains 1 C-type lectin domain. -
结构域
The tandem repeat domain, also called neck domain, mediates oligomerization. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 30835 Human
- Omim: 604672 Human
- SwissProt: Q9NNX6 Human
- Unigene: 278694 Human
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别名
- C type lectin domain family 4 member L antibody
- C-type lectin domain family 4 member L antibody
- CD 209 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody (ab5716)Immunohistochemistry of DC-SIGN in human small intestine tissue with DC-SIGN antibody at 10 µg/ml.
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ab5716 at 2
ab5716 at 2 µg /ml staining approximately 44kDa of human CD209 in Human placenta tissue lysate by Western blot (ECL). Lane 1 absence of blocking peptide and Lane 2 presence of blocking peptide.µ g /ml staining approximately 44kDa of human CD209 in Human placenta tissue lysate by Western blot (ECL). Lane 1 absence of blocking peptide and Lane 2 presence of blocking peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab5716 被引用在 3 文献中.
- Shponka V et al. Frequent expression of activation-induced cytidine deaminase in diffuse large B-cell lymphoma tissues from persons living with HIV. AIDS 34:2025-2035 (2020). PubMed: 32773475
- Zhang L et al. Nab-paclitaxel is an active drug in preclinical model of pediatric solid tumors. Clin Cancer Res 19:5972-83 (2013). Human . PubMed: 23989978
- Mikulak J et al. DC-specific ICAM-3-grabbing nonintegrin mediates internalization of HIV-1 into human podocytes. Am J Physiol Renal Physiol 299:F664-73 (2010). WB ; Human . PubMed: 20630938