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重组RabMAb

重组Anti-DBI抗体[EPR28926-2] - BSA and Azide free (ab317824)

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Western blot - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Western blot - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Western blot - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
  • Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28926-2] to DBI - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

概述

  • 产品名称

    Anti-DBI抗体[EPR28926-2] - BSA and Azide free
    参阅全部 DBI 一抗

  • 描述

    兔单克隆抗体[EPR28926-2] to DBI - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt (Intra),ICC/IF or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse liver, Mouse kidney, Mouse cerebellum, Human liver, Human cerebellum, Rat spleen tissue lysate, McA-RH7777 (rat liver epithelial cell) whole cell lysates , Rat liver, Rat cerebellum, Rat kidney, Mouse skeletal muscle tissue lysates. IHC-P: Human liver, Human cerebrum, Human thyroid carcinoma, Mouse liver, Mouse kidney, Mouse glioblastoma, Rat liver, human spleen, rat spleen, mouse spleen tissues.

  • 常规说明

    ab317824 is the carrier-free version of ab317823

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317824于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

靶标

  • 功能

    Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

  • 组织特异性

    Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.

  • 序列相似性

    Belongs to the ACBP family.
    Contains 1 ACB (acyl-CoA-binding) domain.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ACBD 1 antibody
    • ACBD1 antibody
    • ACBP antibody
    • ACBP_HUMAN antibody
    • Acyl CoA binding domain containing 1 antibody
    • Acyl CoA binding protein antibody
    • Acyl Coenzyme A binding domain containing 1 antibody
    • Acyl coenzyme A binding protein antibody
    • Acyl-CoA-binding protein antibody
    • CCK RP antibody
    • CCKRP antibody
    • Cholecystokinin releasing peptide trypsin sensitive antibody
    • DBI antibody
    • Diazepam-binding inhibitor antibody
    • Endozepine antibody
    • EP antibody
    • GABA receptor modulator antibody
    • MGC70414 antibody
    see all

图片

  • Western blot - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Western blot - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    All lanes : Anti-DBI antibody [EPR28926-2] (ab317823) at 1/1000 dilution

    Lane 1 : McA-RH7777 (rat liver epithelial cell) whole cell lysate
    Lane 2 : Rat liver tissue lysate
    Lane 3 : Rat cerebellum tissue lysate
    Lane 4 : Rat kidney tissue lysate
    Lane 5 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 11 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: spleen (PMID: 2171047).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

  • Western blot - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Western blot - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    All lanes : Anti-DBI antibody [EPR28926-2] (ab317823) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human cerebellum tissue lysate
    Lane 3 : Human spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 11 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: spleen (PMID: 2171047).

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade  (ab176842) staining at 1/100000 dilution.

  • Western blot - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Western blot - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    All lanes : Anti-DBI antibody [EPR28926-2] (ab317823) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse cerebellum tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Mouse skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 11 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: spleen, skeletal muscle (PMID: 2171047).

    The identity of the higher MW band at approximately 25 kDa (in lane 4) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling DBI with ab317823 at 1/4000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: weak staining on rat spleen. The section was incubated with ab317823 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling DBI with ab317823 at 1/4000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: weak staining on mouse spleen. The section was incubated with ab317823 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling DBI with ab317823 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: weak staining on human spleen. The section was incubated with ab317823 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling DBI with ab317823 at 1/4000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat liver. The section was incubated with ab317823 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse glioblastoma tissue labeling DBI with ab317823 at 1/4000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse glioblastoma. The section was incubated with ab317823 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling DBI with ab317823 at 1/4000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse kidney. The section was incubated with ab317823 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling DBI with ab317823 at 1/4000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse liver. The section was incubated with ab317823 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling DBI with ab317823 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human thyroid carcinoma. The section was incubated with ab317823 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling DBI with ab317823 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cerebrum. The section was incubated with ab317823 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

    This data was developed using ab317823, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DBI with ab317823 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human liver. The section was incubated with ab317823 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)
    Anti-DBI antibody [EPR28926-2] - BSA and Azide free (ab317824)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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