Anti-DB1抗体[ZNF5J141] (ab50970)
![Western blot - Anti-DB1 antibody [ZNF5J141] (ab50970)](https://www.abcam.cn/ps/products/50/ab50970/Images/ab50970-62772-anti-db1-antibody-znf5j141-western-blot.gif "Western blot - Anti-DB1 antibody [ZNF5J141] (ab50970)")
Key features and details
- Mouse monoclonal [ZNF5J141] to DB1
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-DB1抗体[ZNF5J141]
参阅全部 DB1 一抗 -
描述
小鼠单克隆抗体[ZNF5J141] to DB1 -
宿主
Mouse -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human DB1.
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阳性对照
- HeLa cells and whole cell lysate and NIH3T3 whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Dibasic monohydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab50970 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
克隆
单克隆 -
克隆编号
ZNF5J141 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50970于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
|
|
| ICC |
1/1.
|
| 说明 |
|---|
|
WB
1/50. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa). |
|
ICC
1/1. |
靶标
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功能
Possible transcription factor. Specifically binds to the CT/GC-rich region of the interleukin-3 promoter and mediates tax transactivation of IL-3. -
组织特异性
Ubiquitously expressed. Highest levels in skeletal muscle and kidney. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7716 Human
- Entrez Gene: 22344 Mouse
- Omim: 606747 Human
- SwissProt: Q14119 Human
- Unigene: 463569 Human
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别名
- DB1 antibody
- MGC82370 antibody
- Putative transcription factor DB1 antibody
see all
图片
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Western blot - Anti-DB1 antibody [ZNF5J141] (ab50970)Anti-DB1 antibody [ZNF5J141] (ab50970) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry - Anti-DB1 antibody [ZNF5J141] (ab50970)](https://www.abcam.cn/ps/products/50/ab50970/Images/ab50970-62771-anti-db1-antibody-znf5j141-immunocytochemistry.gif)
Immunocytochemistry - Anti-DB1 antibody [ZNF5J141] (ab50970)ab50970 at 1/1 dilution staining human DB1 in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat Anti-mouse IgG at 1/200 as secondary.
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![Western blot - Anti-DB1 antibody [ZNF5J141] (ab50970)](https://www.abcam.cn/ps/products/50/ab50970/Images/ab50970-62770-anti-db1-antibody-znf5j141-western-blot.gif)
Western blot - Anti-DB1 antibody [ZNF5J141] (ab50970)Anti-DB1 antibody [ZNF5J141] (ab50970) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab50970 被引用在 2 文献中.
- Fu J et al. Inhibition of miR-495 Improves Both Vascular Remodeling and Angiogenesis in Pulmonary Hypertension. J Vasc Res 56:97-106 (2019). PubMed: 31030195
- Gerald D et al. RhoB controls coordination of adult angiogenesis and lymphangiogenesis following injury by regulating VEZF1-mediated transcription. Nat Commun 4:2824 (2013). WB . PubMed: 24280686
