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Cell Biology Apoptosis Extracellular Signals Death Ligands

Anti-Daxx抗体(ab2017)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (2)

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Immunocytochemistry/ Immunofluorescence - Anti-Daxx antibody (ab2017)

    Key features and details

    • Rabbit polyclonal to Daxx
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Daxx抗体
      参阅全部 Daxx 一抗
    • 描述

      兔多克隆抗体to Daxx
    • 宿主

      Rabbit
    • 经测试应用

      适用于: ICC/IFmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Synthetic peptide corresponding to Human Daxx aa 722-740.
      Sequence:

      TSVATQCDPEEIIVLSDSD


      (Peptide available as ab7879)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.2
      Preservative: 0.02% Sodium azide
    • Concentration information loading...
    • 纯度

      DEAE-Chromatography
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cell Biology
      • Apoptosis
      • Extracellular Signals
      • Death Ligands
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Epigenetics and Nuclear Signaling
      • Chromatin Remodeling
      • Histone chaperones

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Daxx protein (ab131785)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2017于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    Use a concentration of 10 µg/ml.
    说明
    ICC/IF
    Use a concentration of 10 µg/ml.

    靶标

    • 功能

      Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activation of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3.3. Acts as targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upon neuronal activation associates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML-NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3.3 into chromatin. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes; the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3.3 and H4 and decreases binding of CTCF to chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction activity towards human cytomegalovirus (HCMV).
    • 组织特异性

      Ubiquitous.
    • 序列相似性

      Belongs to the DAXX family.
    • 结构域

      The Sumo interaction motif mediates Sumo binding, and is required both for sumoylation and binding to sumoylated targets.
    • 翻译后修饰

      Sumoylated with SUMO1 on multiple lysine residues.
      Phosphorylated by HIPK1 upon glucose deprivation.
      Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation. Ubiquitinated by MDM2; inducing its degradation. Deubiquitinated by USP7; leading to stabilize it.
    • 细胞定位

      Nucleus. Diffuse nuclear distribution pattern and no comparable dot-like accumulation of isoform 1 and Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body. Nucleus, nucleolus. Chromosome, centromere. Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli (Probable). Colocalizes with histone H3.3, ATRX, HIRA and ASF1A at PML-nuclear bodies (PubMed:12953102, PubMed:14990586, PubMed:23222847, PubMed:24200965). Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres (PubMed:9645950). Detected in cytoplasmic punctate structures (PubMed:11842083). Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress (PubMed:12968034). Colocalizes with RASSF1 in the nucleus (PubMed:18566590). Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures (PubMed:16845383).
    • Target information above from: UniProt accession Q9UER7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 1616 Human
      • Omim: 603186 Human
      • SwissProt: Q9UER7 Human
      • Unigene: 336916 Human
      • 别名

        • BING 2 antibody
        • BING2 antibody
        • CENP-C binding protein antibody
        • DAP 6 antibody
        • DAP6 antibody
        • Daxx antibody
        • DAXX_HUMAN antibody
        • Death associated protein 6 antibody
        • Death domain associated protein 6 antibody
        • Death domain associated protein antibody
        • Death domain-associated protein 6 antibody
        • EAP 1 antibody
        • EAP1 antibody
        • ETS1 associated protein 1 antibody
        • ETS1-associated protein 1 antibody
        • Fas binding protein antibody
        • Fas death domain associated protein antibody
        • Fas death domain-associated protein antibody
        • hDaxx antibody
        • MGC126245 antibody
        • MGC126246 antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-Daxx antibody (ab2017)
        Immunocytochemistry/ Immunofluorescence - Anti-Daxx antibody (ab2017)

        ab2017 at 10µg/ml staining DAXX in Hela cells by ICC/IF

      实验方案

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab2017?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2017 被引用在 2 文献中.

      • Lee JS & Zhang Z O-linked N-acetylglucosamine transferase (OGT) interacts with the histone chaperone HIRA complex and regulates nucleosome assembly and cellular senescence. Proc Natl Acad Sci U S A 113:E3213-20 (2016). PubMed: 27217568
      • Dionne KR  et al. Daxx upregulation within the cytoplasm of reovirus-infected cells is mediated by interferon and contributes to apoptosis. J Virol 87:3447-60 (2013). PubMed: 23302889

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-3 of 3 Abreviews or Q&A

      Question

      what is the smallest amount (in nanogrammes) of protein you can detect with ab2017 in western blot analysis? does ab2017 antibody recognizes the denatured AND the native c-terminal part of daxx? does this antibody recognizes (non-specific) proteins from Hela cells? background?

      Read More

      Abcam community

      Verified customer

      Asked on Sep 06 2001

      Answer

      The sensitivity of Western blot is 1-10 ng of protein per band. We detected other antibodies but not this one. It detects the denatured protein, but we have not tested the native protein. It is possible to cross-recognize other proteins.

      Read More

      Neil Hayward

      Abcam Scientific Support

      回复于 Sep 11 2001

      Question

      Can we use this antibody in immunocytochemistry??

      Read More

      Abcam community

      Verified customer

      Asked on Dec 13 2000

      Answer

      This antibody has not been tested for use in immunocytochemistry. It has only been confirmed for Western Blot.

      Read More

      Abcam Scientific Support

      回复于 Dec 14 2000

      Question

      Does this antibody immunoprecipitates?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 17 2000

      Answer

      It has not been tested for immunoprecipitation.

      Read More

      Abcam Scientific Support

      回复于 Nov 17 2000

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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