重组Anti-DARPP32抗体[EP721Y] (ab40802)
![Western blot - Anti-DARPP32 antibody [EP721Y] (ab40802)](https://www.abcam.cn/ps/products/40/ab40802/Images/ab40802-258910-anti-darpp32-antibody-ep721y-western-blot.jpg "Western blot - Anti-DARPP32 antibody [EP721Y] (ab40802)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP721Y] to DARPP32
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DARPP32抗体[EP721Y]
参阅全部 DARPP32 一抗 -
描述
兔单克隆抗体[EP721Y] to DARPP32 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-P, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human brain lysate and human gastric adenocarcinoma. SH-SY5Y (human neuroblastoma) and human fetal forebrain tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.11% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP721Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40802于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/100.
|
|
| WB |
1/5000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Unpurified format: 1/5,000 - 1/20,000 |
|
| IHC-P |
1/50 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IP |
1/50.
|
| 说明 |
|---|
|
ICC/IF
1/50 - 1/100. |
|
WB
1/5000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Unpurified format: 1/5,000 - 1/20,000 |
|
IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/50. |
靶标
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功能
Inhibitor of protein-phosphatase 1. -
序列相似性
Belongs to the protein phosphatase inhibitor 1 family. -
翻译后修饰
Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 84152 Human
- Omim: 604399 Human
- SwissProt: Q9UD71 Human
- Unigene: 286192 Human
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别名
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
see all
图片
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Western blot - Anti-DARPP32 antibody [EP721Y] (ab40802)Anti-DARPP32 antibody [EP721Y] (ab40802) at 1/50000 dilution + Human brain tissue lysates. at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 32 kDa
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)](https://www.abcam.cn/ps/products/40/ab40802/Images/ab40802-258909-anti-darpp32-antibody-ep721y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)ab40802 staining DARPP32 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.
Negative control 1: PBS in place of primary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP721Y] (ab40802)](https://www.abcam.cn/ps/products/40/ab40802/Images/ab40802-258908-anti-darpp32-antibody-ep721y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP721Y] (ab40802)ab40802 staining DARPP32 in SH-SY5Y (human neuroblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab40802 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)](https://www.abcam.cn/ps/products/40/ab40802/Images/ab40802-54010-anti-darpp32-antibody-ep721y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)Immunohistochemical analysis human gastric adenocarcinoma using ab40802. (dilution 1:250) -
![Immunoprecipitation - Anti-DARPP32 antibody [EP721Y] (ab40802)](https://www.abcam.cn/ps/products/40/ab40802/Images/ab40802-258907-anti-darpp32-antibody-ep721y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DARPP32 antibody [EP721Y] (ab40802)ab40802 immunoprecipitating DARPP32. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/50 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.
Lane 1: Human fetal forebrain whole cell lysate (10ug)
Lane 2: Human fetal forebrain whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab40802 in Human fetal forebrain whole cell lysate -
![Anti-DARPP32 antibody [EP721Y] (ab40802)](https://www.abcam.cn/ps/products/40/ab40802/Images/ab40802-7-benefits-of-recombinant-antibodies.png)
Anti-DARPP32 antibody [EP721Y] (ab40802)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab40802 被引用在 3 文献中.
- Sanders B et al. Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants. Nat Commun 13:27 (2022). PubMed: 35031607
- Lange J et al. Mislocalization of Nucleocytoplasmic Transport Proteins in Human Huntington's Disease PSC-Derived Striatal Neurons. Front Cell Neurosci 15:742763 (2021). PubMed: 34658796
- André EM et al. A Combinatorial Cell and Drug Delivery Strategy for Huntington's Disease Using Pharmacologically Active Microcarriers and RNAi Neuronally-Committed Mesenchymal Stromal Cells. Pharmaceutics 11:N/A (2019). PubMed: 31614758