Name: Anti-DARPP32 antibody [EP720Y]
Brand: RabMAb
SKU: ab40801
Rating: 4 (11 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (ab40801)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP720Y] to DARPP32
Suitable for: ICC/IF, WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Carrier Free

产品名称

Anti-DARPP32抗体[EP720Y]
参阅全部 DARPP32 一抗
描述

兔单克隆抗体[EP720Y] to DARPP32
宿主

Rabbit
经测试应用

适用于: ICC/IF, WB, IHC-P, IPmore details
种属反应性

与反应: Mouse, Rat, Human
预测可用于: Pig
免疫原

Synthetic peptide within Human DARPP32 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UD71
(Peptide available as ab189245)
阳性对照
- IHC-P: Human breast adenocarcinoma tissue and colon tissue. Mouse and rat cerebral cortex tissue. ICC/IF: Mouse brain tissue. WB: Rat hippocampus and brain tissue lysate. Mouse cerebral cortex tissue lysate. Human fetal brain lysate. IP: Rat brain whole cell lysate. IHC-Wm: Mouse brain tissue. IHC-Fr: Pig brain tissue.
常规说明
Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) is a protein that in humans is encoded by the PPP1R1B gene. This gene, also known as DARPP-32, has a role as a dopamine- and cyclic AMP-regulated phosphoprotein. As such PPP1R1B affects dopamine glutamate and adenosine; and potentially has a role in conditions such as schizophrenia and Parkinson's disease.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EP720Y
同种型

IgG
研究领域
- Neuroscience
- Processes

Alternative Versions
- Anti-DARPP32 antibody [EP720Y] - BSA and Azide free (ab220808)
- Alexa Fluor® 568 Anti-DARPP32 antibody [EP720Y] (ab313029)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Rat brain cerebrum tissue lysate - total protein (ab4033)

The Abpromise guarantee

Abpromise™承诺保证使用ab40801于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
ICC/IF	(2)	1/50 - 1/100.
WB	(3)	1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). For unpurified use at 1/5000 - 1/50 000.
IHC-P		1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
IP		1/20. For unpurified use at 1/50.

说明
ICC/IF 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). For unpurified use at 1/5000 - 1/50 000.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
IP 1/20. For unpurified use at 1/50.

功能

Inhibitor of protein-phosphatase 1.
序列相似性

Belongs to the protein phosphatase inhibitor 1 family.
翻译后修饰

Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.
细胞定位

Cytoplasm.
Target information above from: UniProt accession Q9UD71 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 84152 Human
- Entrez Gene: 19049 Mouse
- Entrez Gene: 360616 Rat
- Omim: 604399 Human
- SwissProt: Q9UD71 Human
- SwissProt: Q60829 Mouse
- SwissProt: Q6J4I0 Rat
- Unigene: 286192 Human
- Unigene: 45372 Mouse
- Unigene: 70366 Rat
see all
别名
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein antibody
- Dopamine and cAMP regulated phosphoprotein antibody
- Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
- Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
- Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
- FLJ20940 antibody
- IPPD antibody
- Neuronal phosphoprotein DARPP 32 antibody
- PPP1R1B antibody
- PPR1B_HUMAN antibody
- Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
- Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
- Protein phosphatase 1 regulatory subunit 1B antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (ab40801)

Immunohistochemical analysis of paraffin-embedded human colon sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-DARPP32 antibody [EP720Y] (ab40801)

All lanes : Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/50000 dilution (purified)

Lane 1 : Rat hippocampus tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Mouse cerebral cortex tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Blocking/Diluting buffer 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP720Y] (ab40801)

Immunocytochemistry/Immunofluorescence analysis of mouse brain tissue labelling DARPP32 with purified ab40801 at 1/100 (3.4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was performed with Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody.

Secondary Only Control: PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Immunoprecipitation - Anti-DARPP32 antibody [EP720Y] (ab40801)

ab40801 at 1/20 immunoprecipitating DARPP32 in rat brain whole cell lysate observed at 32 KDa (lanes 1 and 2).

Lane 1: Rat brain whole cell lysate 10μg.

Lane 2: ab40801 IP in rat brain whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab40801 in rat brain whole cell lysate.

For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection (1/1000).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (ab40801)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-DARPP32 antibody [EP720Y] (ab40801)

Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/1000 dilution (purified) + Human fetal brain lysate at 15 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Blocking/Diluting buffer 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (ab40801)

Immunohistochemical analysis of human breast adenocarcinoma sections labelling DARPP32 with unpurified ab40801 at a dilution of 1/50.
Western blot - Anti-DARPP32 antibody [EP720Y] (ab40801)

Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/50000 dilution (unpurified) + Rat brain lysate at 10 µg

Predicted band size: 32 kDa
Observed band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (ab40801)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Anti-DARPP32 antibody [EP720Y] (ab40801)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab40801？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab40801 被引用在 96 文献中.

Morelli KH et al. An RNA-targeting CRISPR-Cas13d system alleviates disease-related phenotypes in Huntington's disease models. Nat Neurosci 26:27-38 (2023). PubMed: 36510111
Gangwani MR et al. Neuronal and astrocytic contributions to Huntington's disease dissected with zinc finger protein transcriptional repressors. Cell Rep 42:111953 (2023). PubMed: 36640336
Miura Y et al. Engineering brain assembloids to interrogate human neural circuits. Nat Protoc 17:15-35 (2022). PubMed: 34992269
Palminha NM et al. Defective repair of topoisomerase I induced chromosomal damage in Huntington's disease. Cell Mol Life Sci 79:160 (2022). PubMed: 35224690
Song H et al. Suppression of toxicity of the mutant huntingtin protein by its interacting compound, desonide. Proc Natl Acad Sci U S A 119:e2114303119 (2022). PubMed: 35238684

View all Publications for this product

客户评价及客户问答

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1-10 of 12 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Differentiated NPC)

Permeabilization

Yes - Triton X-100

Specification

Differentiated NPC

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 22 2021

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Brain (Hippocampus/entorhinal))

Permeabilization

Yes - Citrate Buffer, Triton X-100

Specification

Brain (Hippocampus/entorhinal)

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

SRA. PATRICIA VILLANUEVA ANGUITA

Verified customer

提交于 Nov 17 2021

Application

Immunohistochemistry free floating

Sample

Mouse Tissue sections (Brain, 45 µm VIBRATOME sections)

Specification

Brain, 45 µm VIBRATOME sections

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 07 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (human)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Treatment

alpha synuclein

Specification

human

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Sehee Oh

Verified customer

提交于 Jul 13 2016

Application

Flow Cytometry

Sample

Human Cell (Differentiated hNSCs)

Permeabilization

Yes - 0.25% Triton X-100 in DPBS

Gating Strategy

Undifferentiated Stem Cells (white)

Specification

Differentiated hNSCs

Preparation

Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS

Fixation

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 18 2015

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Sample

Human Cell (Differentiated hNSCs)

Specification

Differentiated hNSCs

Permeabilization

Yes - 0.25% Triton X-100 in DPBS

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 17 2015

Application

Immunohistochemistry (Frozen sections)

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Sample

Sheep Tissue sections (Brain Median Eminence)

Specification

Brain Median Eminence

Permeabilization

Yes - 0.01% Triton

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Gregroy Conductier

Verified customer

提交于 Oct 24 2014

Application

IHC - Wholemount

Sample

Zebra finch Tissue (14um brain section)

Specification

14um brain section

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 13 2014

Application

Western blot

Loading amount

10 µg

Gel Running Conditions

Reduced Denaturing (12% Tris Glycine)

Sample

Zebra finch Tissue lysate - other (Brain cytoplasmic fraction)

Specification

Brain cytoplasmic fraction

Blocking step

Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 14 2013

Question

Thank you so much for helping me find primary (and secondary) antibodies for IHC on mouse neural progenitors down the lineage of cortex verses striatum. Attached is the paper (2012) that mentions cortex being identified with NCAM (Figure 4 i,j page 481) and Figure 1 says cortical induction efficiency was measured by Pax6, Vimentin, Otx1 and Otx2 expression (Figure 1c, page 478). The second paper I attached (2008) is to help us figure out a good primary/secondary antibody detection of striatal differentiation. It says “Terminal striatal differentiation can be established by the detection ofMAP-2/Ki67[1] postmitotic neurons expressing key striatal markers, such as GABA, GAD67 (GAD1), DARPP32, ARPP21, calbindin, or calretinin.” If you can find anything more efficient and specific for cortex vs. striatum it would be wonderful! Please contact me for any reason and thank you for your time and energy,

Abcam community

Verified customer

Asked on May 23 2012

Answer

Thank you for contacting us.

While there a many targets which express differentially in distinct layers of the neocortex many of these are expressed in the striatum as well. I found these papers to have a number of good targets which seem specific, such as Pax6 or, for striatal markers, DARPP32, CalB or GAD67. However a search through the Allen Mouse Brain Atlas expression data shows similar gene expression of all these markers in adult striatum and cortex. I would emphasize that this is not vetted data.

While any single or combination of these markers may well yield the information which you will be looking for, I feel that the best choices may be to try a few of these targets presented within this literature as you would have reference material to compare with.

Further review of our catalog leads me to suggest use of a DARPP32 antibody such as ab40801 alongside use of a Vementin antibody such as ab8978. Alternatively use of PAX6 ab5790 alongside GAD67, ab26116, may yield good results.

Each of these pairs will work in double staining as they are comprised of a mouse and a rabbit derived antibody. Further each product has been used in numerous publications and is well characterized by these, Abreviews and scientific support on the datasheet. Each is guaranteed to work in IHC-P as well as a number of other applications and would therefore be covered by our Abpromise guarantee:https://www.abcam.com/abpromise.

I have added links to each of these products below for your review:

ab40801;https://www.abcam.com/DARPP32-antibody-EP720Y-ab40801.html

ab8978;https://www.abcam.com/Vimentin-antibody-RV202-Neural-Stem-Cell-Marker-ab8978.html

ab5790;https://www.abcam.com/PAX6-antibody-Stem-Cell-Marker-ab5790.html

ab26116;https://www.abcam.com/GAD67-antibody-K-87-Neuronal-Marker-ab26116.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Abcam Scientific Support

回复于 May 23 2012

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (96)

客户评价及客户问答

Immunocytochemistry/ Immunofluorescence abreview for Anti-DARPP32 antibody [EP720Y]

Immunohistochemistry (Frozen sections) abreview for Anti-DARPP32 antibody [EP720Y]

Immunohistochemistry free floating abreview for Anti-DARPP32 antibody [EP720Y]

Western blot abreview for Anti-DARPP32 antibody [EP720Y]

Flow Cytometry abreview for Anti-DARPP32 antibody [EP720Y]

Immunocytochemistry/ Immunofluorescence abreview for Anti-DARPP32 antibody [EP720Y]

Immunohistochemistry (Frozen sections) abreview for Anti-DARPP32 antibody [EP720Y]

IHC - Wholemount abreview for Anti-DARPP32 antibody [EP720Y]

Western blot abreview for Anti-DARPP32 antibody [EP720Y]