重组Anti-DARC抗体[EPR5205] (ab137044)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5205] to DARC
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DARC抗体[EPR5205]
参阅全部 DARC 一抗 -
描述
兔单克隆抗体[EPR5205] to DARC -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal liver, Human fetal kidney, Human tonsil, kidney, stomach, TF 1 and K562 lysates; IHC-P: Human kidney tissue IP: TF-1 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5205 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137044于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
1/10 - 1/100.
|
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
|
说明 |
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IP
1/10 - 1/100. |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
靶标
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功能
Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. -
组织特异性
Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium. -
序列相似性
Belongs to the G-protein coupled receptor Duffy family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2532 Human
- Omim: 110700 Human
- SwissProt: Q16570 Human
- Unigene: 153381 Human
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别名
- ACKR1 antibody
- Atypical chemokine receptor 1 antibody
- CCBP1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling DARC with Purified ab137044 at 1:500 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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ab137044 (purified) at 1:30 dilution (2µg) immunoprecipitating DARC in TF-1 whole cell lysate.
Lane 1 (input): TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10µg
Lane 2 (+): ab137044 & TF-1 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137044 in TF-1 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Anti-DARC antibody [EPR5205] (ab137044) at 1/2000 dilution (Purified) + Human kidney lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution (Purified)
Lane 1 : Human stomach lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling DARC with ab137044 at 1/100 dilution.
This image was generated using the unpurified version of the product.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human tonsil lysate
Lane 4 : TF 1 lysate
Lane 5 : K562 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 35 kDaThis image was generated using the unpurified version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab137044 被引用在 3 文献中.
- Hu Z et al. Single-Cell Transcriptomic Atlas of Different Human Cardiac Arteries Identifies Cell Types Associated With Vascular Physiology. Arterioscler Thromb Vasc Biol 41:1408-1427 (2021). PubMed: 33626908
- Wan W et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res 106:478-87 (2015). IHC-Fr ; Mouse . PubMed: 25858253
- Al-Alwan LA et al. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines. J Immunol 193:1416-26 (2014). PubMed: 24981451