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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome

Anti-DAP3抗体(ab2637)

  • Datasheet
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Western blot - Anti-DAP3 antibody (ab2637)
  • Immunocytochemistry/ Immunofluorescence - Anti-DAP3 antibody (ab2637)
  • Western blot - Anti-DAP3 antibody (ab2637)
  • Western blot - Anti-DAP3 antibody (ab2637)

Key features and details

  • Goat polyclonal to DAP3
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-DAP3抗体
    参阅全部 DAP3 一抗
  • 描述

    山羊多克隆抗体to DAP3
  • 宿主

    Goat
  • 特异性

    This antibody is expected to recognise both human isoforms of this protein.
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human DAP3 aa 387-398 (C terminal).
    Sequence:

    NPSLLERHCAYL


    (Peptide available as ab22922)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: HeLa and HepG2 cell lysate and human kidney tissue lysate. ICC/IF: MCF7 cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial translation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human DAP3 protein (ab131869)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2637于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 3 - 10 µg/ml.
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 46 kDa.

1 hour primary incubation is recommended for this product.

说明
ICC/IF
Use a concentration of 3 - 10 µg/ml.
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 46 kDa.

1 hour primary incubation is recommended for this product.

靶标

  • 功能

    Involved in mediating interferon-gamma-induced cell death.
  • 组织特异性

    Ubiquitous.
  • 细胞定位

    Mitochondrion.
  • Target information above from: UniProt accession P51398 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7818 Human
    • Omim: 602074 Human
    • SwissProt: P51398 Human
    • Unigene: 516746 Human
    • 别名

      • 28S ribosomal protein S29 antibody
      • 28S ribosomal protein S29 mitochondrial antibody
      • bMRP10 antibody
      • DAP 3 antibody
      • DAP-3 antibody
      • Dap3 antibody
      • Death associated protein 3 antibody
      • Death-associated protein 3 antibody
      • DKFZp686G12159 antibody
      • FLJ12817 antibody
      • Ionizing radiation resistance conferring protein antibody
      • MGC126058 antibody
      • MGC126059 antibody
      • Mitochondrial 28S ribosomal protein S29 antibody
      • mitochondrial antibody
      • MITOCHONDRIAL RIBOSOMAL PROTEIN S29 antibody
      • MRP S29 antibody
      • MRP-S29 antibody
      • MRPS29 antibody
      • OTTHUMP00000033480 antibody
      • OTTHUMP00000033481 antibody
      • PSD-95/SAP90-binding protein 3 antibody
      • RT29_HUMAN antibody
      • S29mt antibody
      • SAP90/PSD-95-associated protein 3 antibody
      • SAPAP3 antibody
      see all

    图片

    • Western blot - Anti-DAP3 antibody (ab2637)
      Western blot - Anti-DAP3 antibody (ab2637)
      Anti-DAP3 antibody (ab2637) at 0.3 µg/ml + Human kidney tissue lysate in RIPA buffer at 30 µg

      Predicted band size: 46 kDa



      Detected using chemiluminescence.

    • Immunocytochemistry/ Immunofluorescence - Anti-DAP3 antibody (ab2637)
      Immunocytochemistry/ Immunofluorescence - Anti-DAP3 antibody (ab2637)

      Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling DAP3 with ab2637 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.

      Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).

    • Western blot - Anti-DAP3 antibody (ab2637)
      Western blot - Anti-DAP3 antibody (ab2637)
      All lanes : Anti-DAP3 antibody (ab2637) at 0.3 µg/ml

      Lane 1 : HeLa cell lysate in RIPA buffer
      Lane 2 : HepG2 cell lysate in RIPA buffer

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 46 kDa



      Detected using chemiluminescence.

    • Western blot - Anti-DAP3 antibody (ab2637)
      Western blot - Anti-DAP3 antibody (ab2637)
      All lanes : Anti-DAP3 antibody (ab2637) at 1 µg/ml (for 1 hour)

      Lane 1 : HeLa cell lysate in RIPA buffer - control
      Lane 2 : HeLa cell lysate in RIPA buffer - after siRNA mediated DAP3 knock-down expression

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 46 kDa



      Level of knock-down relative to actin expression level determined by RT-PCR.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab2637?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2637 被引用在 1 文献中.

    • Han J  et al. Suppression of adenosine-to-inosine (A-to-I) RNA editome by death associated protein 3 (DAP3) promotes cancer progression. Sci Adv 6:eaba5136 (2020). PubMed: 32596459

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