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AB283703

重组Anti-DAP12抗体[EPR24244-76] - BSA and Azide free

Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal DAP12 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 1 publication.

查看别名

Dap12, Karap, Tyrobp, TYRO protein tyrosine kinase-binding protein, DNAX-activation protein 12, Killer-activating receptor-associated protein, KAR-associated protein

10 Images
Flow Cytometry (Intracellular) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)

This data was developed using ab283679, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse blood cells cells labelling DAP12 with ab283679 at 1/50 dilution (1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. A Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)

This data was developed using ab283679, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling DAP12 with ab283679 at 1/2000 (0.232 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on kupffer cells of rat liver. The section was incubated with ab283679 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)

This data was developed using ab283679, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labelling DAP12 with ab283679 at 1/2000 (0.232 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat lung. The section was incubated with ab283679 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)

This data was developed using ab283679, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling DAP12 with ab283679 at 1/2000 (0.232 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse spleen. The section was incubated with ab283679 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)

This data was developed using ab283679, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling DAP12 with ab283679 at 1/2000 (0.232 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on kupffer cells of mouse liver. The section was incubated with ab283679 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)

This data was developed using ab283679, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized RAW264.7 cells labelling DAP12 with ab283679 at 1/50 (9.28 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in RAW 264.7 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Negative control : M1 (PMID : 11034384).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

Flow Cytometry (Intracellular) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)

This data was developed using ab283679, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized M1 (mouse myeloid leukemia myeloblast) (Left), RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) (Right) cells labelling DAP12 with ab283679 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody.

Negative control : M1 (PMID : 11034384).

Immunoprecipitation - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)
  • IP

Supplier Data

Immunoprecipitation - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)

This data was developed using ab283679, the same antibody clone in a different buffer formulation.

DAP12 was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug with ab283679 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283679 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : RAW264.7 whole cell lysate 10 ug

Lane 2 : ab283679 IP in RAW264.7 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab283679 in RAW264.7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5.5 seconds

All lanes:

Immunoprecipitation - Anti-DAP12 antibody [EPR24244-76] (<a href='/products/primary-antibodies/dap12-antibody-epr24244-76-ab283679'>ab283679</a>)

Predicted band size: 12 kDa

false

Western blot - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)
  • WB

Lab

Western blot - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)

This data was developed using ab283679, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : M1 (PMID : 11034384).

Exposure time : 114 seconds

All lanes:

Western blot - Anti-DAP12 antibody [EPR24244-76] (<a href='/products/primary-antibodies/dap12-antibody-epr24244-76-ab283679'>ab283679</a>) at 1/1000 dilution

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

N9 (mouse microglia), whole cell lysate at 20 µg

Lane 3:

M1 (mouse myeloid leukemia myeloblast), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 12 kDa

false

Western blot - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)
  • WB

Lab

Western blot - Anti-DAP12 antibody [EPR24244-76] - BSA and Azide free (AB283703)

This data was developed using ab283679, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-DAP12 antibody [EPR24244-76] (<a href='/products/primary-antibodies/dap12-antibody-epr24244-76-ab283679'>ab283679</a>) at 1/1000 dilution

All lanes:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

不同偶联物与剂型 (3)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24244-76

亚型

IgG

不含载体蛋白

Yes

反应种属

Rat, Mouse

应用

IHC-P, WB, IP, Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

ab283703 is the carrier-free version of ab283679.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Adapter protein which non-covalently associates with activating receptors found on the surface of a variety of immune cells to mediate signaling and cell activation following ligand binding by the receptors (PubMed : 15471863, PubMed : 9647200). TYROBP is tyrosine-phosphorylated in the ITAM domain following ligand binding by the associated receptors which leads to activation of additional tyrosine kinases and subsequent cell activation (PubMed : 15728241, PubMed : 16715077). Also has an inhibitory role in some cells (PubMed : 21727189). Non-covalently associates with activating receptors of the CD300 family to mediate cell activation (By similarity). Also mediates cell activation through association with activating receptors of the CD200R family (PubMed : 15471863). Required for neutrophil activation mediated by integrin (PubMed : 17086186). Required for the activation of myeloid cells mediated by the CLEC5A/MDL1 receptor (By similarity). Associates with natural killer (NK) cell receptors such as the KLRD1/KLRC2 heterodimer to mediate NK cell activation (By similarity). Also associates non-covalently with the NK cell receptors KLRA4/LY49D and KLRA8/LY49H which leads to NK cell activation (PubMed : 9647200). Associates with TREM1 to mediate activation of neutrophils and monocytes (By similarity). Associates with TREM2 on monocyte-derived dendritic cells to mediate up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (By similarity). Association with TREM2 mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed : 18957693). Stabilizes the TREM2 C-terminal fragment (TREM2-CTF) which is produced by TREM2 ectodomain shedding (By similarity). In microglia, required with TREM2 for phagocytosis of apoptotic neurons (PubMed : 15728241). Required with ITGAM/CD11B in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (PubMed : 18685038). Promotes pro-inflammatory responses in microglia following nerve injury which accelerates degeneration of injured neurons (PubMed : 25690660). Positively regulates the expression of the IRAK3/IRAK-M kinase and IL10 production by liver dendritic cells and inhibits their T cell allostimulatory ability (PubMed : 21257958). Negatively regulates B cell proliferation (PubMed : 21727189). Required for CSF1-mediated osteoclast cytoskeletal organization (PubMed : 18691974). Positively regulates multinucleation during osteoclast development (PubMed : 12569157, PubMed : 14969392).
See full target information Tyrobp
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 3:203-220 PubMed39196190

2024

Cholesterol lowering depletes atherosclerotic lesions of smooth muscle cell-derived fibromyocytes and chondromyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Carramolino,Julián Albarrán-Juárez,Anton Markov,Esther Hernández-SanMiguel,Diana Sharysh,Vanessa Cumbicus,Daniel Morales-Cano,Verónica Labrador-Cantarero,Peter Loof Møller,Paula Nogales,Alberto Benguria,Ana Dopazo,Fátima Sanchez-Cabo,Carlos Torroja,Jacob F Bentzon
View all publications
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