Anti-DAGLA抗体(ab81984)
Key features and details
- Goat polyclonal to DAGLA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DAGLA抗体 -
描述
山羊多克隆抗体to DAGLA -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
C-PAKQDELVISAR
, corresponding to C terminal amino acids 1031-1042 of Human DAGLA. -
阳性对照
- WB: Human liver lysate. IHC-P: Human breast, pancreas, and spleen tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81984于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).
1 hour primary incubation is recommended for this product. |
| IHC-P | (3) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses. -
组织特异性
Highly expressed in brain and pancreas. -
疾病相关
Defects in DAGLA may be a cause of spinocerebellar ataxia type 20 (SCA20) [MIM:608687]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA20 is an autosomal dominant, adult-onset form characterized by dysarthria due to spasmodic dysphonia followed by slowly progressive ataxia. Note=A copy number variation consisting of a 260-kb duplication at chromosome 11q12.2-12.3 is responsible for SCA20. The critical gene within the duplicated segment may be DAGLA. -
序列相似性
Belongs to the AB hydrolase superfamily. Lipase family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 747 Human
- SwissProt: Q9Y4D2 Human
- Unigene: 241564 Human
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别名
- C11orf11 antibody
- Chromosome 11 open reading frame 11 antibody
- DAGL(ALPHA) antibody
see all
图片
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Western blot - Anti-DAGLA antibody (ab81984)Anti-DAGLA antibody (ab81984) at 0.3 µg/ml + human liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 115 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 55 kDa. We are unsure as to the identity of these extra bands.Some minor background is detected and is blocked by the immunizing peptide.
Primary incubation for 1 hour. Detected by chemiluminescence.
实验方案
数据表及文件
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Datasheet download
文献 (8)
ab81984 被引用在 8 文献中.
- Hinden L et al. Opposite physiological and pathological mTORC1-mediated roles of the CB1 receptor in regulating renal tubular function. Nat Commun 13:1783 (2022). PubMed: 35379807
- Mitra S et al. A role for the endocannabinoid enzymes monoacylglycerol and diacylglycerol lipases in cue-induced cocaine craving following prolonged abstinence. Addict Biol 26:e13007 (2021). PubMed: 33496035
- Yoon S et al. cAMP Signaling-Mediated Phosphorylation of Diacylglycerol Lipase α Regulates Interaction With Ankyrin-G and Dendritic Spine Morphology. Biol Psychiatry 90:263-274 (2021). PubMed: 34099188
- Xue SS et al. Repetitive high-frequency transcranial magnetic stimulation reverses depressive-like behaviors and protein expression at hippocampal synapses in chronic unpredictable stress-treated rats by enhancing endocannabinoid signaling. Pharmacol Biochem Behav 184:172738 (2019). PubMed: 31229467
- Smaga I et al. Brain region-dependent changes in the expression of endocannabinoid-metabolizing enzymes in rats following antidepressant drugs. J Physiol Pharmacol 70:N/A (2019). PubMed: 31889042
- Okubo Y et al. Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression. Exp Cell Res 367:112-118 (2018). WB . PubMed: 29614312
- Smaga I et al. Changes in the Brain Endocannabinoid System in Rat Models of Depression. Neurotox Res 31:421-435 (2017). PubMed: 28247204
- Hutch CR et al. An endocannabinoid system is present in the mouse olfactory epithelium but does not modulate olfaction. Neuroscience 300:539-53 (2015). WB ; Mouse . PubMed: 26037800
