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Anti-DAD1抗体(ab23836)

Reviews (1)Q&A (1)References (2)
Western blot - Anti-DAD1 antibody (ab23836)
  • Immunocytochemistry - Anti-DAD1 antibody (ab23836)

Key features and details

  • Rabbit polyclonal to DAD1
  • Suitable for: WB, ICC
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-DAD1抗体

  • 描述

    兔多克隆抗体to DAD1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICCmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to a 14 amino acid sequence within the C terminal region of DAD 1 (Human).

  • 阳性对照

    • HepG2 cell lysates.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Purified by affinity chromatography.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab23836于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa).
ICC
Use at an assay dependent dilution.
说明
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa).
ICC
Use at an assay dependent dilution.

靶标

  • 功能

    Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.

  • 序列相似性

    Belongs to the DAD/OST2 family.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • DAD 1 antibody
      • DAD-1 antibody
      • dad1 antibody
      • DAD1_HUMAN antibody
      • Defender against cell death 1 antibody
      • Dolichyl diphosphooligosaccharide protein glycosyltransferase subunit DAD1 antibody
      • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 antibody
      • Oligosaccharyl transferase subunit DAD1 antibody
      • OST 2 antibody
      • OST2 antibody
      see all

    图片

    • Western blot - Anti-DAD1 antibody (ab23836)
      Western blot - Anti-DAD1 antibody (ab23836)
      Lane 1 : Anti-DAD1 antibody (ab23836) at 0.5 µg/ml
      Lane 2 : Anti-DAD1 antibody (ab23836) at 1 µg/ml
      Lane 3 : Anti-DAD1 antibody (ab23836) at 2 µg/ml

      All lanes : HepG2 whole cell lysate

      Predicted band size: 12 kDa
      Observed band size: 22 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry - Anti-DAD1 antibody (ab23836)
      Immunocytochemistry - Anti-DAD1 antibody (ab23836)
      ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.

    文献 (2)

    发表研究结果有使用 ab23836？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab23836 被引用在 2 文献中.

    • Ding Y  et al. The endoplasmic reticulum-based acetyltransferases, ATase1 and ATase2, associate with the oligosaccharyltransferase to acetylate correctly folded polypeptides. J Biol Chem 289:32044-55 (2014). WB . PubMed: 25301944
    • Murooka TT  et al. CCL5 promotes proliferation of MCF-7 cells through mTOR-dependent mRNA translation. Biochem Biophys Res Commun 387:381-6 (2009). WB ; Human . PubMed: 19607806

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-DAD1 antibody

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (skin)
    Loading amount
    15 µg
    Specification
    skin
    Gel Running Conditions
    Non-reduced Denaturing (15% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 24 2009

    Question

    I am thinking of purchasing DAD1 antibody to use against Xenopus Laevis. The primary accession number is P46967, and the fasta format follows. >sp|P46967|DAD1_XENLA Defender against cell death 1 (DAD-1) - Xenopus laevis (African clawed frog). MSVSVFSVVSRFLDEYVSSTPQRLKLLDAYLLYILLTGALQFLYCLLVGTFPFNSFLSGFISSVGSFILAVCLRIQINPQNKSDFQGISPERAFADFLFANTILHLVVVNFIG I was wondering if you could give me an idea of the chance that your anti body will detect this version of the protein? Thank you

    Read More
    Answer

    Thank you for your enquiry. I contacted the antibody originator regarding ab23836. Unfortunately the amino acid sequence of the immunogen is proprietary. The only information I have is that the sequence was selected from the last half of the protein, but not the very C-terminus. The amino acid sequence of human DAD1 is in the public domain (GenBank accession no. AAH09798). However, without access to the immunogen sequence I am not in a position to comment on the homology between the relevant sequence in human versus xenopus. I am sorry that I could not assist further. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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