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AB23836

Anti-DAD1抗体

Anti-DAD1 antibody

5

(1 Review)

|

(2 Publications)

Rabbit Polyclonal DAD1 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DAD1.

查看别名

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Oligosaccharyl transferase subunit DAD1, Defender against cell death 1, DAD-1, DAD1

2 Images
Immunocytochemistry - Anti-DAD1 antibody (AB23836)
  • ICC

Unknown

Immunocytochemistry - Anti-DAD1 antibody (AB23836)

ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.

Western blot - Anti-DAD1 antibody (AB23836)
  • WB

Unknown

Western blot - Anti-DAD1 antibody (AB23836)

Lane 1:

Western blot - Anti-DAD1 antibody (ab23836) at 0.5 µg/mL

Lane 2:

Western blot - Anti-DAD1 antibody (ab23836) at 1 µg/mL

Lane 3:

Western blot - Anti-DAD1 antibody (ab23836) at 2 µg/mL

All lanes:

HepG2 whole cell lysate

Predicted band size: 12 kDa

Observed band size: 22 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC, WB

applications

免疫原

Synthetic Peptide within Human DAD1. The exact immunogen used to generate this antibody is proprietary information.

P61803

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
纯化说明
Purified by affinity chromatography.
存储溶液
Preservative: 0.02% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed : 22467853, PubMed : 31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes. Loss of the DAD1 protein triggers apoptosis (PubMed : 22467853).
See full target information DAD1

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 289:32044-55 PubMed25301944

2014

The endoplasmic reticulum-based acetyltransferases, ATase1 and ATase2, associate with the oligosaccharyltransferase to acetylate correctly folded polypeptides.

Applications

WB

Species

Unspecified reactive species

Yun Ding,Cosma D Dellisanti,Mi Hee Ko,Cynthia Czajkowski,Luigi Puglielli

Biochemical and biophysical research communications 387:381-6 PubMed19607806

2009

CCL5 promotes proliferation of MCF-7 cells through mTOR-dependent mRNA translation.

Applications

WB

Species

Human

Thomas T Murooka,Ramtin Rahbar,Eleanor N Fish
View all publications

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