Anti-DACH1抗体(ab222832)
Key features and details
- Rabbit polyclonal to DACH1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-DACH1抗体
参阅全部 DACH1 一抗 -
描述
兔多克隆抗体to DACH1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse
-
免疫原
Recombinant fragment corresponding to Human DACH1 aa 150-400.
Database link: Q9UI36 -
阳性对照
- IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
>95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222832于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/200.
|
|
| ICC/IF |
1/50 - 1/200.
|
| 说明 |
|---|
|
IHC-P
1/20 - 1/200. |
|
ICC/IF
1/50 - 1/200. |
靶标
-
功能
Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain. -
组织特异性
Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen. -
序列相似性
Belongs to the DACH/dachshund family. -
结构域
The DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 1602 Human
- Entrez Gene: 13134 Mouse
- Omim: 603803 Human
- SwissProt: Q9UI36 Human
- SwissProt: Q9QYB2 Mouse
- Unigene: 129452 Human
- Unigene: 320593 Mouse
-
别名
- DACH antibody
- Dach1 antibody
- DACH1_HUMAN antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DACH1 antibody (ab222832)
Paraffin-embedded human kidney tissue stained for DACH1 using ab222832 at 1/100 dilution in immunohistochemical analysis.
-
Immunocytochemistry/ Immunofluorescence - Anti-DACH1 antibody (ab222832)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for DACH1 (green) using ab222832 at 1/100 dilution in ICC/IF.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab222832 尚未被引用在任何文献中。