Anti-D-glucosamine抗体(ab62666)
Key features and details
- Rabbit polyclonal to D-glucosamine
- Suitable for: ICC
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-D-glucosamine抗体 -
描述
兔多克隆抗体to D-glucosamine -
宿主
Rabbit -
特异性
Fixed tissue cross-reactivity tested with known targets at recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against any free D or L amino acid. The IgG is highly specifc for glucosamine (L/D differences not determined yet). As most other pentoses are unfixable (lacking primary amino groups), this antibody cannot detect them in fixed tissues. -
经测试应用
适用于: ICCmore details -
种属反应性
与反应: Species independent -
免疫原
D-glucosamine cross-linked to purifed fraction V bovine serum albumin with glutaraldehyde.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Thimerosal (merthiolate)
Constituents: PBS, 1% Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62666于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
| 说明 |
|---|
Endogenous content mapping by LM and EM immunocytochemistry. True dilution at user dilution: 1/2000. Optimal fixation: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI (High Performance Immunocytochemistry). Minimum glutaraldehyde: 0.05% using EHPI (Enhanced HPI) with 4% formaldehyde.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性
D-glucosamine, or glucosamine-6-phosphate is the biochemical precursor of all nitrogen-containing sugars. It is synthesized from fructose-6-phosphate and glutamine as the first step of the hexosamine biosynthesis pathway. The end-product of this pathway is UDP-N-acetylglucosamine (UDP-GlcNAc), which is then used for making glycosaminoglycans, proteoglycans, and glycolipids. -
别名
- Glucosamine 6 phosphate antibody
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab62666 被引用在 1 文献中.
- Dai N et al. Evaluation of UDP-GlcN derivatives for selective labeling of 5-(hydroxymethyl)cytosine. Chembiochem 14:2144-52 (2013). PubMed: 24106095