Anti-Cytosolic Phospholipase A2抗体(ab58375)
Key features and details
- Rabbit polyclonal to Cytosolic Phospholipase A2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cytosolic Phospholipase A2抗体
参阅全部 Cytosolic Phospholipase A2 一抗 -
描述
兔多克隆抗体to Cytosolic Phospholipase A2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Cytosolic Phospholipase A2 aa 450-550. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P47712 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab58375 was affinity purified from rabbit antiserum affinity chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58375于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 85 kDa).
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 85 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
组织特异性
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
序列相似性
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
结构域
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
翻译后修饰
Activated by phosphorylation at both Ser-505 and Ser-727. -
细胞定位
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
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数据库链接
- Entrez Gene: 151056 Human
- Entrez Gene: 5321 Human
- Omim: 600522 Human
- SwissProt: P47712 Human
- SwissProt: Q6P1J6 Human
- Unigene: 444933 Human
- Unigene: 497200 Human
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别名
- Calcium dependent phospholipid binding protein antibody
- CPLA 2 antibody
- cPLA2 alpha antibody
see all
图片
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Western blot - Anti-Cytosolic Phospholipase A2 antibody (ab58375)All lanes : Anti-Cytosolic Phospholipase A2 antibody (ab58375) at 1/500 dilution
Lane 1 : HeLa cell extracts treated
with TNF alpha (20ng/ml, 30mins)
Lane 2 : HeLa cell extracts treated
with TNF alpha (20ng/ml, 30mins) with immunising peptide
Predicted band size: 85 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody (ab58375)ab58375 at 1/50-1/100 dilution staining Cytosolic Phospholipase A2 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody (ab58375)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of Human spleen tissue, staining Phospholipase A2 with ab58375.
Tissue was fixed with formaldehyde and blocked with blocking solution for 10 minutes at 37°C; antigen retrieval was by heat mediation in a cell conditioner. Samples were incubated with primary antibody (undiluted) for 1 hour at 37°C. A biotinylated-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab58375 被引用在 22 文献中.
- Sun J et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharm Biol 59:672-682 (2021). PubMed: 34078224
- Wu J & Chen X Acupuncture therapy protects PCOS patients with diabetes by regulating miR-32-3p/PLA2G4A pathway. Am J Transl Res 13:8819-8832 (2021). PubMed: 34539997
- Xylaki M et al. Changes in the cellular fatty acid profile drive the proteasomal degradation of α-synuclein and enhance neuronal survival. FASEB J 34:15123-15145 (2020). PubMed: 32931072
- Xue Y et al. Gigantol ameliorates CCl4-induced liver injury via preventing activation of JNK/cPLA2/12-LOX inflammatory pathway. Sci Rep 10:22265 (2020). PubMed: 33335297
- Ding Y et al. Annexin A1 attenuates neuroinflammation through FPR2/p38/COX-2 pathway after intracerebral hemorrhage in male mice. J Neurosci Res 98:168-178 (2020). PubMed: 31157469