重组Anti-Cytoplasmic Dynein Intermediate chain抗体[74.1] (ab23905)

Blocking buffer and concentration: 5% NFDM/TBST

Exposure time:

Lanes 1-3: 37 seconds
Lanes 4-6: 81 seconds

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling Cytoplasmic Dynein Intermediate chain with ab23905 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) at 1/2000 was used as the secondary antibody (red). Mouse monoclonal IgG was used as the isotype control (black). Cells without incubation with primary and secondary antibodies were used as the unlabeled control (blue). 

Cytoplasmic Dynein Intermediate chain was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 µg with ab23905 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab23905 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.

Lane 1: Rat brain tissue lysate 10 µg

Lane 2: ab23905 IP in Rat brain tissue lysate

Lane 3: Mouse monoclonal IgG instead of ab23905 in rat brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second

Cytoplasmic Dynein Intermediate chain was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10 µg with ab23905 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab23905 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.

Lane 1: U-87 MG whole cell lysate 10 µg

Lane 2: ab23905 IP in U-87 MG whole cell lysate

Lane 3: Mouse monoclonal IgG instead of ab23905 in U-87 MG whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second

Flow Cytometry analysis of C6 (rat glial tumor cell line) cells labeling Cytoplasmic Dynein Intermediate chain with ab23905 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) at 1/2000 was used as the secondary antibody (red). Mouse monoclonal IgG was used as the isotype control (black). Cells without incubation with primary and secondary antibodies were used as the unlabeled control (blue). 

Gel: 10% acryl amid
Blocking Step: 5% Milk for 30 minutes at 25°C

This image was generated from the hybridoma version of the product.

See Abreview