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Microbiology Organism Virus DNA Virus double stranded DNA Virus CMV

Anti-Cytomegalovirus pp65抗体[3A12] - BSA and Azide free (ab6503)

  • Datasheet
Submit a review Q&A (2)References (28)

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Key features and details

  • Mouse monoclonal [3A12] to Cytomegalovirus pp65 - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Human cytomegalovirus
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-Cytomegalovirus pp65抗体[3A12] - BSA and Azide free
    参阅全部 Cytomegalovirus pp65 一抗
  • 描述

    小鼠单克隆抗体[3A12] to Cytomegalovirus pp65 - BSA and Azide free
  • 宿主

    Mouse
  • 特异性

    Cytomegalovirus pp65 (Lower Matrix Protein).
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Human cytomegalovirus
  • 免疫原

    Tissue, cells or virus corresponding to Cytomegalovirus pp65.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Constituent: 100% PBS
  • 无载体

    是
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    3A12
  • 骨髓瘤

    NS1/1-Ag4-1
  • 同种型

    IgG2a
  • 轻链类型

    kappa
  • 研究领域

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • CMV

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Cytomegalovirus pp65 protein (ab43041)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6503于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/16000.
ICC/IF
1/3200.
说明
WB
1/16000.
ICC/IF
1/3200.

靶标

  • 相关性

    Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.
  • 数据库链接

    • Entrez Gene: 1487754 Human cytomegalovirus
    • SwissProt: P06725 Human cytomegalovirus
    • 别名

      • 65 kDa matrix phosphoprotein antibody
      • 65 kDa phosphoprotein antibody
      • Tegument protein pp65 antibody
      • Tegument protein UL83 antibody

    实验方案

    • Immunofluorescence Assay (IFA)
    • Indirect ELISA
    • Western blot for Viruses

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (28)

    发表研究结果有使用 ab6503?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6503 被引用在 28 文献中.

    • Zhang Y  et al. COG133 Attenuates the Early Brain Injury Induced by Blood-Brain Barrier Disruption in Experimental Subarachnoid Hemorrhage. J Healthc Eng 2022:4404039 (2022). PubMed: 35035834
    • Vezzani G  et al. The Human Cytomegalovirus UL116 Glycoprotein Is a Chaperone to Control gH-Based Complexes Levels on Virions. Front Microbiol 12:630121 (2021). PubMed: 33889136
    • Gonzalez-Perez AC  et al. The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts. mBio 12:N/A (2021). PubMed: 33947766
    • Liu M  et al. Ferulic acid inhibits LPS-induced apoptosis in bovine mammary epithelial cells by regulating the NF-κB and Nrf2 signalling pathways to restore mitochondrial dynamics and ROS generation. Vet Res 52:104 (2021). PubMed: 34256834
    • Xu J & Ma X Hsa_circ_0032131 knockdown inhibits osteoarthritis progression via the miR-502-5p/PRDX3 axis. Aging (Albany NY) 13:15100-15113 (2021). PubMed: 34032607
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    Hi, We are interested in antibodies against human CMV pp65. We don't need the antibody for the whole pp65 protein, rather we need the AB for a certain epitope. please send us your info. Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 20 2011

    Answer

    Thank you for your inquiry. As you may have seen already, we have a number of HCMV pp65 antibodies available in our catalog (such as ab6503, ab8250, ab31624, ab49214, ab53489). These were raised against the pp65 protein, however, unfortunately the exact epitope recognised by each antibody has not been mapped, as epitope mapping is not routinely performed.  I am sorry that I cannot be more helpful on this occasion. Please do not hesitate to contact us if you have any further questions.  

    Read More

    Abcam Scientific Support

    回复于 Oct 20 2011

    Question

    I am looking for an antibody to pp65 that will recognize epitopes beyond those found in the terminal third of the pp65 coding sequence - do you know what region of pp65 this antibody recognizes? I do not need specifics, just whether this product will work for me.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    I put your question forward to the originator but they said that the epitope has not been characterised so they do not have the information. I am sorry we cannot be of help to you.

    Read More

    Asdf Edo

    Abcam Scientific Support

    回复于 Oct 13 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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