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Anti-Cytomegalovirus Glycoprotein B抗体[HCMV37] (ab54023)

Submit a review Q&A (2)References (6)
Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] (ab54023)

    Key features and details

    • Mouse monoclonal [HCMV37] to Cytomegalovirus Glycoprotein B
    • Suitable for: ELISA, ICC/IF, IHC-Fr, Neutralising
    • Reacts with: Human cytomegalovirus
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    FITC

    概述

    • 产品名称

      Anti-Cytomegalovirus Glycoprotein B抗体[HCMV37]
      参阅全部 Cytomegalovirus Glycoprotein B 一抗

    • 描述

      小鼠单克隆抗体[HCMV37] to Cytomegalovirus Glycoprotein B

    • 宿主

      Mouse

    • 特异性

      This antibody is specific for Cytomegalovirus glycoprotein B late neutralizing antigen. Reacts with MRC-5 infected cells but not uninfected cells.

    • 经测试应用

      适用于: ELISA, ICC/IF, IHC-Fr, Neutralisingmore details

    • 种属反应性

      与反应: Human cytomegalovirus

    • 免疫原

      Human CMV extracts

    • 阳性对照

      • ICC/IF: monocyte-derived dendritic cells infected with Human cytomegalovirus

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • 存储溶液

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 纯度

      Protein A purified

    • 克隆

      单克隆

    • 克隆编号

      HCMV37

    • 同种型

      IgG2a

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab54023于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ELISA
    ICC/IF
    IHC-Fr
    Neutralising
    说明
    应用说明
    ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution (CMV infected cells).
    IHC-Fr: Use at an assay dependent dilution (CMV infected cells).
    Neut: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    靶标

    • 相关性

      Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.

    • 细胞定位

      Virus envelope.

    • 数据库链接

      • 别名

        • CMV glycoprotein B antibody
        • Glycoprotein B antibody
        • HCMV antibody
        • HHV5 glycoprotein B antibody
        • UL55 antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] (ab54023)
        Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] (ab54023)Image from Haspot F et al., PLoS One. 2012;7(4):e34795. Epub 2012 Apr 9. Fig 2.; doi:10.1371/journal.pone.0034795; April 9, 2012, PLoS ONE 7(4): e34795.
        ab54023, staining Cytomegalovirus Glycoprotein B in monocyte-derived dendritic cells infected with Human cytomegalovirus (VHL/E strain), cultured for 3 hours (left panels) and 24 hours (right panels), by Immunocytochemistry/ Immunofluorescence.

        Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS for 10 minutes at room temperature. Samples were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse monoclonal IgG was used as the secondary antibody.

      文献 (6)

      发表研究结果有使用 ab54023？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab54023 被引用在 6 文献中.

      • Alba-Patiño A  et al. Nanoparticle Reservoirs for Paper-Only Immunosensors. ACS Sens 5:147-153 (2020). PubMed: 31850749
      • Ulasov IV  et al. CMV70-3P miRNA contributes to the CMV mediated glioma stemness and represents a target for glioma experimental therapy. Oncotarget 8:25989-25999 (2017). PubMed: 27517625
      • Wang B  et al. Viperin is induced following toll-like receptor 3 (TLR3) ligation and has a virus-responsive function in human trophoblast cells. Placenta 36:667-73 (2015). PubMed: 25814471
      • Kirchmeier M  et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein B antigen induces antibodies with potent and broad neutralizing activity. Clin Vaccine Immunol 21:174-80 (2014). Neutralising . PubMed: 24334684
      • Haspot F  et al. Human cytomegalovirus entry into dendritic cells occurs via a macropinocytosis-like pathway in a pH-independent and cholesterol-dependent manner. PLoS One 7:e34795 (2012). ICC/IF . PubMed: 22496863
      • Cranage MP  et al. Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus. EMBO J 5:3057-63 (1986). PubMed: 3024973

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Question

      Looking for a neutralization protocol

      Read More
      Answer

      Antibody ab54023 has been tested in a neutralization assay that is described in the following reference. Figure 4 shows the data for this clone.

      Kirchmeier M et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein B antigen induces antibodies with potent and broad neutralizing activity. Clin Vaccine Immunol 21:174-80 (2014).

      We do not test this antibody in a neutralization assay in-house so I do not have a protocol to send to you. A much simpler assay then the one used in the Kirchmeier paper is briefly described in the following paper. The assay involves counting PFU after incubation of virus with HCMV gB antiserum. The assay is described in the last paragraph of the paper. It does not mention applying this test to this clone or other monoclonal antibodies but it seems like an easy approach.

      EMBO J. 1986 Nov; 5(11): 3057–3063.

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1167261/?page=1

      The other HCMV antibody you have, ab6499, has not, to our knowledge, been used in a neutralization assay.

      Read More

      Question

      What was the source of this antibody? Was it derived from tissue culture supernatant or ascites fluid?

      Read More
      Answer

      This antibody was derived from ascites fluid.

      Read More

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