Anti-Cytokeratin 8+18抗体[5D3] (ab17139)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)](https://www.abcam.cn/ps/products/17/ab17139/Images/ab17139-4-anti-cytokeratin-8-18-antibody-5d3-immunohistochemistry-skin-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)")
Key features and details
- Mouse monoclonal [5D3] to Cytokeratin 8+18
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cytokeratin 8+18抗体[5D3]
参阅全部 Cytokeratin 8+18 一抗 -
描述
小鼠单克隆抗体[5D3] to Cytokeratin 8+18 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 8+18. Cytokeratins from the human breast carcinoma cell line MCF-7
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阳性对照
- IHC-P: Human Skin tissue. Flow cyt: MCF7 cells.
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常规说明
This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
Primary antibody说明
This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas. -
克隆
单克隆 -
克隆编号
5D3 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17139于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 21102523
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
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WB
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 21102523 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 3856 Human
- Entrez Gene: 3875 Human
- Omim: 148060 Human
- Omim: 148070 Human
- SwissProt: P05787 Human
- Unigene: 533782 Human
- Unigene: 708445 Human
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别名
- CARD2 antibody
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)Paraffin embedded human skin tissue stained for Cytokeratin 8/18 using ab 17139 in immunohistochemical analysis.
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![Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)](https://www.abcam.cn/ps/products/17/ab17139/Images/ab17139-25954-anti-cytokeratin-818-antibody-5d3-flow-cytometry.jpg)
Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)Overlay histogram showing MCF7 cells stained with ab17139 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17139, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
实验方案
数据表及文件
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Datasheet download
文献 (8)
ab17139 被引用在 8 文献中.
- Kane N et al. Significant changes in macrophage and CD8 T cell densities in primary prostate tumors 2 weeks after SBRT. Prostate Cancer Prostatic Dis 26:207-209 (2023). PubMed: 35058580
- Mihalcioiu C et al. Improved platform for breast cancer circulating tumor cell enrichment and characterization with next-generation sequencing technology. Am J Cancer Res 13:25-44 (2023). PubMed: 36777514
- You Z et al. The Clinical Significance of Hepatic CD69+ CD103+ CD8+ Resident-Memory T Cells in Autoimmune Hepatitis. Hepatology 74:847-863 (2021). PubMed: 33554350
- Yang BY et al. Porous Se@SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species-NF-?B pathway inhibition. Acta Biomater 88:392-405 (2019). PubMed: 30753941
- Bhat V et al. Isolation and characterization of a new basal-like luminal progenitor in human breast tissue. Stem Cell Res Ther 10:269 (2019). PubMed: 31443683
- Ramachandra Rao S et al. Compromised phagosome maturation underlies RPE pathology in cell culture and whole animal models of Smith-Lemli-Opitz Syndrome. Autophagy 14:1796-1817 (2018). PubMed: 29979914
- Xu D et al. Enabling autologous human liver regeneration with differentiated adipocyte stem cells. Cell Transplant 23:1573-84 (2014). PubMed: 24148223
- Wang Y et al. Transforming growth factor-ß regulates the sphere-initiating stem cell-like feature in breast cancer through miRNA-181 and ATM. Oncogene : (2010). ICC/IF ; Human . PubMed: 21102523
