Anti-Cytokeratin 8抗体[C-43] (ab2530)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)](https://www.abcam.cn/ps/products/2/ab2530/Images/ab2530-194376-anti-cytokeratin-8-antibody-c-43-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)")
Key features and details
- Mouse monoclonal [C-43] to Cytokeratin 8
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-Cytokeratin 8抗体[C-43]
参阅全部 Cytokeratin 8 一抗 -
描述
小鼠单克隆抗体[C-43] to Cytokeratin 8 -
宿主
Mouse -
特异性
This antibody recognises the 52.5kDa Cytokeratin 8. -
经测试应用
适用于: WB, ICC/IF, IHC-P, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Sheep, Rabbit, Cow, Pig
不与反应:
Mouse, Rat, Chicken, Hamster, Xenopus laevis -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 8. Cytoskeleton preparation from HeLa human cervix carcinoma cell line
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阳性对照
- IHC-P: Human lung FFPE tissue sections. ICC/IF: HepG2 cells WB: HeLa cell lysate Flow Cyt (Intra): MCF7 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from tissue culture supernatant. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
C-43 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 52.5 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 10 µg/ml.
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| Flow Cyt (Intra) |
Use a concentration of 1 - 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 52.5 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 10 µg/ml. |
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Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
疾病相关
Cirrhosis -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
细胞定位
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 281269 Cow
- Entrez Gene: 3856 Human
- Entrez Gene: 100152077 Pig
- Entrez Gene: 100348891 Rabbit
- Entrez Gene: 101110495 Sheep
- Omim: 148060 Human
- SwissProt: P05786 Cow
- SwissProt: P05787 Human
see all -
别名
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)
IHC image of ab2530 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2530, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [C-43] (ab2530)](https://www.abcam.cn/ps/products/2/ab2530/Images/ab2530-5920-anti-cytokeratin-8-antibody-c-43-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [C-43] (ab2530)ICC/IF image of ab2530 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2530, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Western blot - Anti-Cytokeratin 8 antibody [C-43] (ab2530)](https://www.abcam.cn/ps/products/2/ab2530/Images/ab2530-331853-anti-cytokeratin-8-antibody-c-43-western-blot.jpg)
Western blot - Anti-Cytokeratin 8 antibody [C-43] (ab2530)Anti-Cytokeratin 8 antibody [C-43] (ab2530) at 2 µg/ml + HeLA cell lysate
Predicted band size: 52.5 kDa -
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)](https://www.abcam.cn/ps/products/2/ab2530/Images/ab2530-195391-anti-cytokeratin-8-antibody-c-43-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)Overlay histogram showing MCF7 cells stained with ab2530 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2530, 0.1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab2530 被引用在 9 文献中.
- Kim SY et al. Sodium butyrate inhibits high cholesterol-induced neuronal amyloidogenesis by modulating NRF2 stabilization-mediated ROS levels: involvement of NOX2 and SOD1. Cell Death Dis 11:469 (2020). PubMed: 32555166
- Yamazaki Y et al. PAX1 is essential for development and function of the human thymus. Sci Immunol 5:N/A (2020). PubMed: 32111619
- Ekoue DN et al. GPX1 Localizes to the Nucleus in Prostate Epithelium and its Levels are not Associated with Prostate Cancer Recurrence. Antioxidants (Basel) 7:N/A (2018). PubMed: 30453672
- Canciello A et al. Progesterone prevents epithelial-mesenchymal transition of ovine amniotic epithelial cells and enhances their immunomodulatory properties. Sci Rep 7:3761 (2017). IHC-P ; Sheep . PubMed: 28630448
- Volpi S et al. EXTL3 mutations cause skeletal dysplasia, immune deficiency, and developmental delay. J Exp Med 214:623-637 (2017). PubMed: 28148688
- Elakoum R et al. CARM1 and PRMT1 are dysregulated in lung cancer without hierarchical features. Biochimie 97:210-8 (2014). WB ; Human . PubMed: 24211191
- Barboni B et al. Synthetic bone substitute engineered with amniotic epithelial cells enhances bone regeneration after maxillary sinus augmentation. PLoS One 8:e63256 (2013). ICC/IF ; Sheep . PubMed: 23696804
- Impens F et al. Mechanistic insight into taxol-induced cell death. Oncogene 27:4580-91 (2008). WB ; Human . PubMed: 18408750
- Bártek J et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol 164:215-24 (1991). PubMed: 1716305