Anti-Cytokeratin 8抗体(ab175249)
Key features and details
- Rabbit polyclonal to Cytokeratin 8
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Cytokeratin 8抗体
参阅全部 Cytokeratin 8 一抗 -
描述
兔多克隆抗体to Cytokeratin 8 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow
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免疫原
Recombinant full length protein corresponding to Human Cytokeratin 8 aa 1-483.
Sequence:MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGL GGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNN KFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQ LETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDV DEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDN SRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDL RRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDAN AKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEES RLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSS SFSRTSSSRAVVVKKIETRDGKLVSESSDV LPK
Database link: P05787 -
阳性对照
- 293T, HeLa, DU145 and NIH3T3 cell line lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175249于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/500 - 1/2000. Predicted molecular weight: 66 kDa.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/500 - 1/2000. Predicted molecular weight: 66 kDa. |
靶标
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功能
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
疾病相关
Cirrhosis -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
细胞定位
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 281269 Cow
- Entrez Gene: 3856 Human
- Entrez Gene: 16691 Mouse
- Omim: 148060 Human
- SwissProt: P05786 Cow
- SwissProt: P05787 Human
- SwissProt: P11679 Mouse
- Unigene: 533782 Human
see all -
别名
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab175249)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling Cytokeratin 8 with ab175249 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (ab175249)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling Cytokeratin 8 with ab175249 at 1/200. Magnification: 400x. -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (ab175249)Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab175249. Blue DAPI for nuclear staining. -
Western blot - Anti-Cytokeratin 8 antibody (ab175249)All lanes : Anti-Cytokeratin 8 antibody (ab175249) at 1/500 dilution
Lane 1 : 293T cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : DU145 cell line lysate
Lane 4 : NIH3T3 cell line lysate
Predicted band size: 66 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab175249 尚未被引用在任何文献中。