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Anti-Cytokeratin 8 + 18 + 19抗体[2A4] (ab41825)

Reviews (1)Q&A (4)References (21)
Flow Cytometry - Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)

Key features and details

  • Mouse monoclonal [2A4] to Cytokeratin 8 + 18 + 19
  • Suitable for: WB, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Rat, Human, Pig
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Cytokeratin 8 + 18 + 19抗体[2A4]

  • 描述

    小鼠单克隆抗体[2A4] to Cytokeratin 8 + 18 + 19

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, IHC-Fr, IHC-P, ICC/IF, Flow Cytmore details

  • 种属反应性

    与反应: Rat, Human, Pig

  • 免疫原

    Full length native protein (purified) corresponding to Pig Cytokeratin 8 + 18 + 19.

  • 常规说明

    Balb/c spleen cells used to produce hybridoma.


    This antibody recognizes cytokeratins 8, 18 and 19 typical for simple epithelial cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • 存储溶液

    pH: 7.3
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • 纯度

    Protein G purified

  • Primary antibody说明

    This antibody recognizes cytokeratins 8, 18 and 19 typical for simple epithelial cells.

  • 克隆

    单克隆

  • 克隆编号

    2A4

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab41825于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (1)
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab91353 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
WB
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab91353 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性

    The cytokeratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. Keratin proteins belong to 2 families: acidic (or type I) and basic (or type II). The acidic keratins are coded by genes KRT9 to 19, the basic keratins by genes KRT1 to 8. Cytokeratins 8, 18 and 19 are encoded by the genes KRT8, 18 and 19 respectively. Type I and II keratins are usually coordinately synthesized in preferential pairs so that at least 1 member of each family is expressed in each epithelial cell, in equal proportions. Cytokeratin 8, together with its filament partner cytokeratin 18, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues. KRT8/18 mutations may predispose to liver disease, including cryptogenic cirrhosis, in humans. Unlike its related family members, cytokeratin 19 (the smallest known acidic cytokeratin at 40kDa) is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. It is often expressed in cultured epithelial cells and in some carcinomas.

  • 细胞定位

    Cytoplasmic

  • 数据库链接

    see all

  • 别名

    • CK18 antibody
    • CK19 antibody
    • CK8 antibody
    • K18 antibody
    • K19 antibody
    • K8 antibody
    • keratin 18 antibody
    • keratin 19 antibody
    • keratin 8 antibody
    • Keratin type I 40kDa antibody
    • Keratin type I cytoskeletal 18 antibody
    • Keratin type I cytoskeletal 19 antibody
    • Keratin type II cytoskeletal 8 antibody
    • KRT18 antibody
    • KRT19 antibody
    • KRT8 antibody
    see all

图片

  • Flow Cytometry - Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)
    Flow Cytometry - Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)
    Overlay histogram showing MCF7 cells stained with ab41825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41825, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 + 18 + 19 antibody [2A4] (ab41825)
    IHC image of ab41825 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41825, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献 (21)

发表研究结果有使用 ab41825？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab41825 被引用在 21 文献中.

  • Hsiao HY  et al. Delivery and Transcriptome Assessment of an In Vitro Three-Dimensional Proximal Tubule Model Established by Human Kidney 2 Cells in Clinical Gelatin Sponges. Int J Mol Sci 24:N/A (2023). PubMed: 37958530
  • Sung H  et al. High-sucrose diet exposure is associated with selective and reversible alterations in the rat peripheral taste system. Curr Biol 32:4103-4113.e4 (2022). PubMed: 35977546
  • Gening SO  et al. The Detection of Stem-Like Circulating Tumor Cells Could Increase the Clinical Applicability of Liquid Biopsy in Ovarian Cancer. Life (Basel) 11:N/A (2021). PubMed: 34440558
  • Rangel-Pozzo A  et al. Genomic Analysis of Localized High-Risk Prostate Cancer Circulating Tumor Cells at the Single-Cell Level. Cells 9:N/A (2020). PubMed: 32784507
  • Yang Y  et al. MiR-135 suppresses glycolysis and promotes pancreatic cancer cell adaptation to metabolic stress by targeting phosphofructokinase-1. Nat Commun 10:809 (2019). PubMed: 30778058
View all Publications for this product

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1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8 + 18 + 19 antibody [2A4]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF-7)
Specification
MCF-7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton-X 100
Blocking step
Serum as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 24 2012

Question

Customer is interested in ab41825, but wonders if we could label this one for him. Suggested ab102849 to do it himself to save money.
Will the 1% BSA interfer with the labeling process? Unfortunately, yes. ab128745 is necessary (or a pull down is perfomed within the lab).

Read More
Answer

Vielen Dank für Ihren Anruf.

Leider sind 0,5% BSA das Maximum, dass das Labeling-Kit toleriert. Daher wäre entweder eine IP notwendig, mit der Sie den Antikörper von BSA aufreinigen notwendig, oder aber Sie benutzen ein weiteres Kit priori dem eigentlichen Labeling Kit.

Zusammenfassend lässt sich sagen dass Sie die folgenden Produkte von uns zur Konjugation erwerben können:

https://www.abcam.com/Cytokeratin-8--18--19-antibody-2A4-ab41825.html (or use the following: https://www.abcam.com/Cytokeratin-8--18--19-antibody-2A4-ab41825.html).

https://www.abcam.com/EasyLink-Alkaline-phosphatase-Conjugation-Kit-1-x-100g-AP-ab102849.html (or use the following: https://www.abcam.com/EasyLink-Alkaline-phosphatase-Conjugation-Kit-1-x-100g-AP-ab102849.html).

https://www.abcam.com/Mouse-Antibody-Purification-Kit-ab128745.html (or use the following: https://www.abcam.com/Mouse-Antibody-Purification-Kit-ab128745.html).





Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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Question

I was wondering if I could get the expiration dates of two primary antibodies please:


Cytokeratin 8+18+19, ab41825, stored @ 2-8 C

GGT1, ab55138, stored also @ 2-8 C.

Thanks so much.

Read More
Answer

Thank you for contacting us.

While we do not provide explicit expiration dates for our antibodies, we will guaranteethemfor six months from the date of purchase when stored according to our datasheet instructions. For ab41825 and ab55138, we would not recommend storage at 2-8C for longer than 1-2 weeks. For maximum stability, these antibodies should be frozen and repeated freeze / thaw cycles should be avoided. When frozen, the antibodies should be stable for at least one year, possibly 2-3 years.

I hope this helps, please let me know if you need any additional information or assistance.

Read More

Question

I would like to test ab41825 for use in ICC.

Read More
Answer

DISCOUNT CODE: ***
Expiration date: October 19th, 2012

I am very pleased to hear you would like to accept our offer and test ab41825 in ICC. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question

Inquiry: Dear Sir/ Madam, One customer raised an inquiry about ab118855(Anti-Keratin 8+18+19 antibody [2A4]) and ab41825 (Anti-Cytokeratin 8 + 18 + 19 antibody [2A4]). She found that these 2 antibodies are both for Cytokeratin 8 + 18 + 19 and raised from the same clone: 2A4. Therefore, she would like to ask whether these 2 antibodies are the same antibody. Beside, which antibody is recommended for ICC/IF? Would you please help her resolve the problems? Thank you very much. Best regards,

Read More
Answer

Thank you for your enquiry regarding ab41825 and ab118855.

We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. These two products fall into the latter category. The identity of our suppliers remains commercially sensitive information.

The clone number is the same, though the purification and the testing conditions (ie. tested species, tested applications, positive control) are different. ab11885 has been further characterised so we keep both antibodies in our catalogue.

Currently, we are unable to offer custom-made conjugation to APC and Alexa 647. Apologies!

I hope this helps and if I can assist further, please do not hesitate to contact me.

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