Anti-Cytokeratin 7+17抗体[C-46] (ab670)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.cn/ps/products/0/ab670/Images/ab670-3606-anti-cytokeratin-717-antibody-c-46-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)")
Key features and details
- Mouse monoclonal [C-46] to Cytokeratin 7+17
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Cytokeratin 7+17抗体[C-46] -
描述
小鼠单克隆抗体[C-46] to Cytokeratin 7+17 -
宿主
Mouse -
特异性
Cytokeratin peptides 7 (54 kDa) and 17 (46 kDa) -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
不与反应: Mouse, Rat, Rabbit -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 7+17. Cytoskeleton preparation of HeLa cells (cervix carcinoma)
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阳性对照
- IHC-P: Human Pancreas tissue WB: HeLa
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常规说明
Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from culture supernatant. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
C-46 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml.
Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication, 5 min. in hot Laemmli buffer (reducing conditions) |
|
| IHC-P |
Use a concentration of 10 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB |
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication, 5 min. in hot Laemmli buffer (reducing conditions) |
|
IHC-P
Use a concentration of 10 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB |
靶标
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相关性
Cytokeratin 7 and 17 is a heterotetramer of a type I and type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Cytokeratin 17 is required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 3855 Human
- Entrez Gene: 3872 Human
- SwissProt: P08729 Human
- SwissProt: Q04695 Human
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别名
- CK17 antibody
- CK7 antibody
- Cytokeratin 17 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue.
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![Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.cn/ps/products/0/ab670/Images/ab670-473353-anticytokeratin-717-antibody-c46-western-blot-hela.jpg)
Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)All lanes : Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 2 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Non-Reducing)
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (Non-Reducing)
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Reducing)
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (Reducing)
Secondary
All lanes : IRDye800-conjugated anti-mouse IgG1 secondary antibody
Observed band size: 46,54 kDa why is the actual band size different from the predicted?A specific band was detected for cytokeratin 17 at approximately 46 kDa and for cytokeratin 7 at approximately 54 kDa.
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![Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.cn/ps/products/0/ab670/Images/ab670-3607-anti-cytokeratin-717-antibody-c-46-western-blot.jpg)
Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 1 µg/ml + HeLa cell lysate
Observed band size: 49,51 kDa why is the actual band size different from the predicted?
Sample preparation:Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab670 被引用在 3 文献中.
- Hegstad J et al. Female rat sexual behavior is unaffected by perinatal fluoxetine exposure. Psychoneuroendocrinology 120:104796 (2020). PubMed: 32711369
- Sun Y et al. Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis. Toxicology 262:162-70 (2009). PubMed: 19524636
- Vojtesek B et al. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma 37:333-42 (1990). PubMed: 1695327