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Anti-Cytokeratin 7抗体[RCK105] - Cytoskeleton Marker (ab9021)

Reviews (16)Q&A (9)References (104)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

Key features and details

  • Mouse monoclonal [RCK105] to Cytokeratin 7 - Cytoskeleton Marker
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Human, Pig
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Cytokeratin 7抗体[RCK105] - Cytoskeleton Marker
    参阅全部 Cytokeratin 7 一抗

  • 描述

    小鼠单克隆抗体[RCK105] to Cytokeratin 7 - Cytoskeleton Marker

  • 宿主

    Mouse

  • 特异性

    This antibody reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.

  • 经测试应用

    适用于: IHC-Fr, IHC-Pmore details

  • 种属反应性

    与反应: Human, Pig
    预测可用于: Rat, Goat, Hamster, Cat, Dog, Zebrafish

  • 免疫原

    Full length native protein (purified) corresponding to Human Cytokeratin 7. BALB/c mouse immunized with cytokeratins from the human bladder carcinoma cell line T24.

  • 阳性对照

    • IHC-P: Human liver tissue, mouse ovary and bladder tissue IHC-Fr: Human liver, urinary bladder tissue, Pig liver and skin tissue

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 克隆

    单克隆

  • 克隆编号

    RCK105

  • 骨髓瘤

    Sp2/0-Ag14

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab9021于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr (2)
1/100 - 1/500.
IHC-P (5)
1/1000.
说明
IHC-Fr
1/100 - 1/500.
IHC-P
1/1000.

靶标

  • 功能

    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

  • 组织特异性

    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

  • 序列相似性

    Belongs to the intermediate filament family.

  • 翻译后修饰

    Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CK 7 antibody
      • CK-7 antibody
      • CK7 antibody
      • Cytokeratin 7 antibody
      • Cytokeratin-7 antibody
      • D15Wsu77e antibody
      • K2C7 antibody
      • K2C7_HUMAN antibody
      • K7 antibody
      • Keratin 7 antibody
      • Keratin 7, type II antibody
      • Keratin type II cytoskeletal 7 antibody
      • Keratin, 55K type II cytoskeletal antibody
      • Keratin, simple epithelial antibody
      • Keratin, simple epithelial type I, K7 antibody
      • Keratin, type II cytoskeletal 7 antibody
      • Keratin-7 antibody
      • Krt2-7 antibody
      • KRT7 antibody
      • MGC11625 antibody
      • MGC129731 antibody
      • MGC3625 antibody
      • Sarcolectin antibody
      • SCL antibody
      • Type II mesothelial keratin K7 antibody
      • Type-II keratin Kb7 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

      Immunohistochemistry analysis of formalin fixed, paraffin embedded sections of human liver tissue labeling Cytokeratin 7 with ab9021 at 1/100; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.

    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

      ab9021 staining normal pig skin tissue section (frozen), staining epithelial cells using ab9021 at 1/500.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)Yang, X. et al PLoS One. 2013 Sep 18;8(9):e74809. doi: 10.1371/journal.pone.0074809. eCollection 2013 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunofluorescent staining of β-galactosideasae and cytokeratin expression of urinary bladder after intravesical treatment with Adeno-Cre

      Formalin-fixed, paraffin-embedded mouse bladder tissue stained for β-galactosidase (green, A) or Cytokeratin 7 (red, B). Panel C shows the colocalization of β-galactosidase and Cytokeratin 7. The nuclear counterstain is DAPI (blue).

      Cytokeratin 7 was detected using ab9021.

      (After Figure 3 of Yang et al)

    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

      ab9021 staining normal breast tissue section (frozen), fixated in Methanol, staining epithelial cells. These pictures are part of the review submitted by Rene Villadsen.

    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

      Immunohistochemistry analysis of frozen sections of human liver labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.

    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)

      Immunohistochemistry analysis of frozen sections of human urinary bladder labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells and no reactivity in connective tissue.

    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry on frozen sections of human liver, only bile duct epithelium positive; no reaction in hepatocytes
    • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry on frozen sections of swine liver, only bile duct epithelium positive; no reaction in hepatocytes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)This image is courtesy of an Abreview submitted by These pictures are part of the review submitted by Todd Gocken

      Formalin fixed parrafin embedded mouse ovary tissue. Ab9021 used at 1:1000 incubated with overnight incubation with a biotinylated secondary and peroxidase/DAB detection. 

    文献 (104)

    发表研究结果有使用 ab9021？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab9021 被引用在 104 文献中.

    • Wang F  et al. Claudin18.2 as a potential therapeutic target for primary ovarian mucinous carcinomas and metastatic ovarian mucinous carcinomas from upper gastrointestinal primary tumours. BMC Cancer 23:44 (2023). PubMed: 36639622
    • Pikkusaari S  et al. Functional Homologous Recombination Assay on FFPE Specimens of Advanced High-Grade Serous Ovarian Cancer Predicts Clinical Outcomes. Clin Cancer Res 29:3110-3123 (2023). PubMed: 36805632
    • Zhang XY  et al. IL-27 deficiency inhibits proliferation and invasion of trophoblasts via the SFRP2/Wnt/β-catenin pathway in fetal growth restriction. Int J Med Sci 20:392-405 (2023). PubMed: 36860682
    • Fischer JR  et al. Multiplex imaging of breast cancer lymph node metastases identifies prognostic single-cell populations independent of clinical classifiers. Cell Rep Med 4:100977 (2023). PubMed: 36921599
    • Kitao M  et al. Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker. iScience 26:107016 (2023). PubMed: 37389178
    View all Publications for this product

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 11 2022

    Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Liver)
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 04 2008

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