Anti-Cytokeratin 7抗体(ab53123)
Key features and details
- Rabbit polyclonal to Cytokeratin 7
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cytokeratin 7抗体
参阅全部 Cytokeratin 7 一抗 -
描述
兔多克隆抗体to Cytokeratin 7 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
A synthetic peptide derived from human Cytokeratin 7.
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阳性对照
- HepG2 cell extracts and human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53123 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53123于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| WB |
1/300 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
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WB
1/300 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
组织特异性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Arg-20 is dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3855 Human
- Omim: 148059 Human
- SwissProt: P08729 Human
- Unigene: 411501 Human
- Unigene: 670221 Human
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别名
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody (ab53123)ab53123 at 1/50 dilution staining Cytokeratin 7 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
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Western blot - Anti-Cytokeratin 7 antibody (ab53123)All lanes : Anti-Cytokeratin 7 antibody (ab53123) at 1/300 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide
Predicted band size: 51 kDa
Observed band size: 51 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody (ab53123)ICC/IF image of ab53123 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab53123 被引用在 6 文献中.
- Afshar Y et al. Circulating trophoblast cell clusters for early detection of placenta accreta spectrum disorders. Nat Commun 12:4408 (2021). PubMed: 34344888
- Johnson LJ et al. Human Placental Trophoblasts Infected by Listeria monocytogenes Undergo a Pro-Inflammatory Switch Associated With Poor Pregnancy Outcomes. Front Immunol 12:709466 (2021). PubMed: 34367171
- Azari S et al. Hofbauer Cells Spread Listeria monocytogenes among Placental Cells and Undergo Pro-Inflammatory Reprogramming while Retaining Production of Tolerogenic Factors. mBio 12:e0184921 (2021). PubMed: 34399615
- Ehrenberg KR et al. Systematic Generation of Patient-Derived Tumor Models in Pancreatic Cancer. Cells 8:N/A (2019). PubMed: 30744205
- Rakers S et al. Monitoring changing cellular characteristics during the development of a fin cell line from Cyprinus carpio. Comp Biochem Physiol B Biochem Mol Biol 225:1-12 (2018). PubMed: 29960082
- Ball CR et al. Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts. EMBO Mol Med 9:918-932 (2017). IHC-P, ICC/IF . PubMed: 28526679