Anti-Cytokeratin 5 + 14抗体[LH8] (ab20203)
Key features and details
- Mouse monoclonal [LH8] to Cytokeratin 5 + 14
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Human
- Isotype: IgM
概述
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产品名称
Anti-Cytokeratin 5 + 14抗体[LH8]
参阅全部 Cytokeratin 5 + 14 一抗 -
描述
小鼠单克隆抗体[LH8] to Cytokeratin 5 + 14 -
宿主
Mouse -
特异性
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. -
经测试应用
适用于: IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
The 50,000 Mr band (including K14 )cut from an SDS-polyacrylamide gel and Triton X-100 extracted cytoskeleton pellet from cultured human foreskin keratinocytes.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
LH8 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgM -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20203于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. PubMed: 24705787
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| 说明 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. PubMed: 24705787 |
靶标
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相关性
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. -
细胞定位
Cytoplasmic; Basal layer -
数据库链接
- Entrez Gene: 3852 Human
- Entrez Gene: 3861 Human
- Omim: 148040 Human
- Omim: 148066 Human
- SwissProt: P02533 Human
- SwissProt: P13647 Human
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别名
- CK 14 antibody
- CK 5 antibody
- DDD antibody
see all
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab20203 被引用在 2 文献中.
- Moser B et al. Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology. PLoS One 9:e94118 (2014). IHC-P ; Human . PubMed: 24705787
- Prince ME et al. Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proc Natl Acad Sci U S A 104:973-8 (2007). IHC-Fr ; Human . PubMed: 17210912