Anti-Cytokeratin 20抗体(ab53120)
Key features and details
- Rabbit polyclonal to Cytokeratin 20
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cytokeratin 20抗体
参阅全部 Cytokeratin 20 一抗 -
描述
兔多克隆抗体to Cytokeratin 20 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
A synthetic peptide derived from human Cytokeratin 20.
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阳性对照
- HeLa cell extracts and human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53120于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/300 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/300 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. -
组织特异性
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. -
序列相似性
Belongs to the intermediate filament family. -
发展阶段
First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. -
翻译后修饰
Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 54474 Human
- Entrez Gene: 286912 Rat
- Omim: 608218 Human
- SwissProt: P35900 Human
- SwissProt: P25030 Rat
- Unigene: 84905 Human
- Unigene: 9887 Rat
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别名
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
see all
图片
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Western blot - Anti-Cytokeratin 20 antibody (ab53120)All lanes : Anti-Cytokeratin 20 antibody (ab53120) at 1/300 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with immunising peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody (ab53120)ab53120 at 1/50 dilution staining Cytokeratin 20 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab53120 被引用在 6 文献中.
- Clayton DR et al. Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA+ interstitial cells are fibroblasts. Am J Physiol Renal Physiol 323:F299-F321 (2022). PubMed: 35834272
- Dalghi MG et al. Functional roles for PIEZO1 and PIEZO2 in urothelial mechanotransduction and lower urinary tract interoception. JCI Insight 6:N/A (2021). PubMed: 34464353
- Eikesdal HP et al. BMP7 Signaling in TGFBR2-Deficient Stromal Cells Provokes Epithelial Carcinogenesis. Mol Cancer Res 16:1568-1578 (2018). PubMed: 29934328
- Janardhan KS et al. Immunohistochemistry in Investigative and Toxicologic Pathology. Toxicol Pathol 46:488-510 (2018). PubMed: 29966501
- Kullmann FA et al. Urothelial proliferation and regeneration after spinal cord injury. Am J Physiol Renal Physiol 313:F85-F102 (2017). PubMed: 28331065
- Bhusari S et al. Mutation Spectra of Kras and Tp53 in Urethral and Lung Neoplasms in B6C3F1 Mice Treated with 3,3',4,4'-Tetrachloroazobenzene. Toxicol Pathol N/A:N/A (2013). Mouse . PubMed: 23703846