Anti-Cytokeratin 19抗体[RCK108] (ab9221)
Key features and details
- Mouse monoclonal [RCK108] to Cytokeratin 19
- Suitable for: Flow Cyt, IHC-Fr, IHC-P
- Reacts with: Human, Zebrafish
- Isotype: IgG1
概述
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产品名称
Anti-Cytokeratin 19抗体[RCK108]
参阅全部 Cytokeratin 19 一抗 -
描述
小鼠单克隆抗体[RCK108] to Cytokeratin 19 -
宿主
Mouse -
特异性
This antibody reacts exclusively with cytokeratin 19 which is present in glandular-type epithelia and most carcinomas. It does not react with hepatocytes and hepatocellular carcinoma. -
经测试应用
适用于: Flow Cyt, IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Human, Zebrafish -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 19. Human bladder carcinoma cell line T24.
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常规说明
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
Primary antibody说明
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. -
克隆
单克隆 -
克隆编号
RCK108 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
应用 | Ab评论 | 说明 |
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Flow Cyt |
1/100 - 1/200.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr | (1) |
1/100 - 1/200.
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IHC-P | (1) |
1/100 - 1/200.
|
说明 |
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Flow Cyt
1/100 - 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
1/100 - 1/200. |
IHC-P
1/100 - 1/200. |
靶标
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功能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
序列相似性
Belongs to the intermediate filament family. -
发展阶段
Present in hair follicles at all stages of development. -
结构域
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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数据库链接
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
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别名
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
Indirect immunoperoxidase staining of human colon adenocarcinoma paraffin tissue section with ab9221. Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial tumor cells. No reactivity in connective tissues.
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Immunohistochemistry on a frozen tissue sections of human kidney with ab9221 (1:500).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
Indirect immunoperoxidase staining of human small intestine paraffin tissue section with ab9221. Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.
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Immunohistochemistry on frozen tissue sections of human colon with ab9221 (1:500).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
Indirect immunoperoxidase staining of human placenta paraffin tissue section with ab9221. Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)ab9221 staining Cytokeratin 19 in human skin (sweat glands).
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Overlay histogram showing MCF-7 cells stained with ab9221 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9221, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
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ab9221 staining cytokeratin 19 in three-day-old Zebrafish tissue by Immunohistochemistry (Frozen sections).
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Immunohistochemistry on frozen section of human liver bile duct epithelium
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab9221 被引用在 18 文献中.
- Yang L et al. Trophoblast organoids with physiological polarity model placental structure and function. J Cell Sci 137:N/A (2024). PubMed: 37676312
- Kim JH et al. Integrative analysis of single-cell RNA-seq and ATAC-seq reveals heterogeneity of induced pluripotent stem cell-derived hepatic organoids. iScience 26:107675 (2023). PubMed: 37680467
- Grossman JE et al. Organoid Sensitivity Correlates with Therapeutic Response in Patients with Pancreatic Cancer. Clin Cancer Res 28:708-718 (2022). PubMed: 34789479
- Liu D et al. Spatial Multiomics Analysis Reveals Only Minor Genetic and Epigenetic Changes in Human Liver Cancer Stem-Like Cells Compared With Other Tumor Parenchymal Cells. Front Cell Dev Biol 10:810687 (2022). PubMed: 35223840
- Di Matteo S et al. Therapeutic effects of dexamethasone-loaded hyaluronan nanogels in the experimental cholestasis. Drug Deliv Transl Res 12:1959-1973 (2022). PubMed: 35226290