重组Anti-Cytokeratin 19抗体[EPNCIR127B] (ab133496)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR127B] to Cytokeratin 19
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-Cytokeratin 19抗体[EPNCIR127B]
参阅全部 Cytokeratin 19 一抗 -
描述
兔单克隆抗体[EPNCIR127B] to Cytokeratin 19 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Mouse Cytokeratin 19 aa 350 to the C-terminus (C terminal).
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阳性对照
- Mouse kidney, Mouse heart, Mouse spleen, and F9 cell lysates, Mouse colon tissue
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常规说明
This antibody was developed as part of a collaboration between the National Cancer Institutes Center for Cancer Research and the lab of Terry Van Dyke. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPNCIR127B -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133496于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 44 kDa).
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IHC-P | (4) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 44 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. |
靶标
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功能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
序列相似性
Belongs to the intermediate filament family. -
发展阶段
Present in hair follicles at all stages of development. -
结构域
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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数据库链接
- Entrez Gene: 16669 Mouse
- SwissProt: P19001 Mouse
- Unigene: 439699 Mouse
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别名
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling Cytokeratin 19 with purified ab133496 at 1:500 dilution (1.33 ?g/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-Cytokeratin 19 antibody [EPNCIR127B] (ab133496) at 1/1000 dilution (Purified)
Lane 1 : Mouse kidney lysates
Lane 2 : Mouse heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 44 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of mouse orofacial tissue, staining Cytokeratin 19 with unpurified ab133496.
Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/500 in diluent) for 24 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal IgG (1/2) was used as the secondary antibody. -
Immunohistochemical analysis of paraffin embedded Mouse colon tissue labelling Cytokeratin 19 using unpurified ab133496 antibody at a dilution of 1/100.
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All lanes : Anti-Cytokeratin 19 antibody [EPNCIR127B] (ab133496) at 1/1000 dilution (unpurified)
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lane 4 : F9 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (57)
ab133496 被引用在 57 文献中.
- Schoultz E et al. Tissue architecture delineates field cancerization in BRAFV600E-induced tumor development. Dis Model Mech 15:N/A (2022). PubMed: 34379110
- Datta R et al. Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors. Sci Adv 8:eabg6383 (2022). PubMed: 35061540
- Zhu S et al. Liver Endothelial Heg Regulates Vascular/Biliary Network Patterning and Metabolic Zonation Via Wnt Signaling. Cell Mol Gastroenterol Hepatol 13:1757-1783 (2022). PubMed: 35202885
- Zhou X et al. Deficient Rnf43 potentiates hyperactive Kras-mediated pancreatic preneoplasia initiation and malignant transformation. Animal Model Exp Med 5:61-71 (2022). PubMed: 35229994
- Cao X et al. Establishment of a Novel Mouse Hepatocellular Carcinoma Model for Dynamic Monitoring of Tumor Development by Bioluminescence Imaging. Front Oncol 12:794101 (2022). PubMed: 35251971