重组Anti-Cytokeratin 18抗体[EPR1625] (ab133272)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1625] to Cytokeratin 18
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cytokeratin 18抗体[EPR1625]
参阅全部 Cytokeratin 18 一抗 -
描述
兔单克隆抗体[EPR1625] to Cytokeratin 18 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa and MCF-7 cell lysates, HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1625 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133272于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 48 kDa.
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ICC/IF |
1/500 - 1/1000.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 48 kDa. |
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
组织特异性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
疾病相关
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
细胞定位
Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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别名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
图片
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All lanes : Anti-Cytokeratin 18 antibody [EPR1625] (ab133272) at 1/10000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : MCF-7 cell lysates
Predicted band size: 48 kDa -
Immunofluorescent staining of HeLa cells labelling Cytokeratin 18 using ab133272, at 1/500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133272 被引用在 1 文献中.
- Yang Y et al. FBXL10 promotes EMT and metastasis of breast cancer cells via regulating the acetylation and transcriptional activity of SNAI1. Cell Death Discov 7:328 (2021). PubMed: 34718323