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重组RabMAb

重组Anti-Cytokeratin 18抗体[EPR1611] (ab133302)

Submit a review Q&A (1)References (3)
Western blot - Anti-Cytokeratin 18 antibody [EPR1611] (ab133302)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [EPR1611] (ab133302)
  • Anti-Cytokeratin 18 antibody [EPR1611] (ab133302)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1611] to Cytokeratin 18
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Cytokeratin 18抗体[EPR1611]
    参阅全部 Cytokeratin 18 一抗

  • 描述

    兔单克隆抗体[EPR1611] to Cytokeratin 18

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IFmore details
    不适用于: Flow Cyt,IHC-P or IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • MCF-7, A431, and HeLa whole cell lysate (ab150035), HeLa cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab133302于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/10000 - 1/50000. Predicted molecular weight: 48 kDa.
ICC/IF
1/100 - 1/250.
说明
WB
1/10000 - 1/50000. Predicted molecular weight: 48 kDa.
ICC/IF
1/100 - 1/250.
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.

靶标

  • 功能

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

  • 组织特异性

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

  • 疾病相关

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

  • 序列相似性

    Belongs to the intermediate filament family.

  • 翻译后修饰

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

  • 细胞定位

    Cytoplasm > perinuclear region.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • Cell proliferation-inducing gene 46 protein antibody
      • CK 18 antibody
      • CK-18 antibody
      • CK18 antibody
      • CYK 18 antibody
      • CYK18 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin endo B antibody
      • Cytokeratin-18 antibody
      • K 18 antibody
      • K18 antibody
      • K1C18_HUMAN antibody
      • KA18 antibody
      • Keratin 18 antibody
      • Keratin 18, type I antibody
      • Keratin D antibody
      • keratin, type I cytoskeletal 18 antibody
      • Keratin-18 antibody
      • Krt18 antibody
      see all

    图片

    • Western blot - Anti-Cytokeratin 18 antibody [EPR1611] (ab133302)
      Western blot - Anti-Cytokeratin 18 antibody [EPR1611] (ab133302)
      All lanes : Anti-Cytokeratin 18 antibody [EPR1611] (ab133302) at 1/10000 dilution

      Lane 1 : MCF-7 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : HeLa cell lysates

      Predicted band size: 48 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [EPR1611] (ab133302)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [EPR1611] (ab133302)
      Immunofluorescence analysis of HeLa cells labelled with ab133302 at 1/100 dilution.
    • Anti-Cytokeratin 18 antibody [EPR1611] (ab133302)
      Anti-Cytokeratin 18 antibody [EPR1611] (ab133302)

    文献 (3)

    发表研究结果有使用 ab133302？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab133302 被引用在 3 文献中.

    • Urban L  et al. Unravelling heterogeneous effects of cancer‑associated fibroblasts on poor prognosis markers in breast cancer EM‑G3 cell line: In vitro‑targeted treatment (anti‑IL-6, anti‑VEGF-A, anti‑MFGE8) based on transcriptomic profiling. Oncol Rep 51:N/A (2024). PubMed: 37975220
    • Jeong BY  et al. Oxidative stress caused by activation of NADPH oxidase 4 promotes contrast-induced acute kidney injury. PLoS One 13:e0191034 (2018). PubMed: 29329317
    • Hirai A  et al. Prognostic impact of programmed death-ligand 1 expression in correlation with human leukocyte antigen class I expression status in stage I adenocarcinoma of the lung. J Thorac Cardiovasc Surg 155:382-392.e1 (2018). PubMed: 28711324

    客户评价及客户问答

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    Question

    I'm searching for CK and vimentin antibodies that recognize human proteins but not the corresponding mouse proteins.

    Read More
    Answer

    Although mouse samples were not recommended based on the western blot results below with these antibodies, there is still the possibility that the negative results in mouse might be due to the limited sample types, especially for the antibodies with high homology (more than 80%) with mouse proteins.
    CK 18
    ab32118: waiting homology and mouse testing
    CK 8
    ab32357: waiting homology, blank WB in mouse brain, heart, lung, muscle, spleen
    CK 7
    ab52870: no homology, no mouse testing performed due to no homology
    CK 19
    ab76539: 80% homology, blank WB in mouse brain, heart, kidney, spleen
    CK 18
    ab133272: 93% homology, blank WB in mouse brain, heart, kidney, spleen, NIH/3T3, Raw264.7
    CK 18
    ab133302: 86% homology, blank WB in mouse brain, heart, kidney, spleen, NIH/3T3, Raw 264.7
    Vimentin
    ab133260: 100% homology, blank WB in mouse brain, heart, kidney, spleen. Non-specific bands in NIH/3T3.
    Epcam
    I could not find any antibodies in our catalog that have low homology with mouse. Your best option is to look for an antibody with an immunogen between human Epcam aa 36-53, the least homologous region of the protein with mouse Epcam. We do provide custom antibody creation in which we can create a rabbit monoclonal antibody with a synthetic peptide in a region of your choosing, please let me know if this is something you are interested in, and I'd be happy to put you in contact with our custom antibody services group.

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