Anti-Cytokeratin 18抗体[DC 10] (ab7797)
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)](https://www.abcam.cn/ps/products/7/ab7797/Images/ab7797-471155-ab7797-anti-cytokeratin-18-antibody-human-immunocytochemistry.png "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)")
Key features and details
- Mouse monoclonal [DC 10] to Cytokeratin 18
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Cytokeratin 18抗体[DC 10]
参阅全部 Cytokeratin 18 一抗 -
描述
小鼠单克隆抗体[DC 10] to Cytokeratin 18 -
宿主
Mouse -
特异性
Specific reactivity for the cytokeratin 18 (45 kD) polypeptide. -
经测试应用
适用于: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Human
不与反应: Mouse, Rat, Sheep, Hamster, Cow, Dog, Pig -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 18. PMC-42 human breast carcinoma cells.
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阳性对照
- ICC/IF: Hep-2 cells. IHC-P: Human colon carcinoma. WB: HeLa, HT-29 cell lysate. Flow Cyt (Intra): MCF-7 cells; HeLa cells.
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常规说明
This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from TCS -
Primary antibody说明
This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. -
克隆
单克隆 -
克隆编号
DC 10 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml.
|
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| ICC/IF | (7) |
1/400.
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| Flow Cyt (Intra) |
Use a concentration of 6 - 16 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 10 µg/ml. |
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ICC/IF
1/400. |
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Flow Cyt (Intra)
Use a concentration of 6 - 16 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB
Use a concentration of 1 - 2 µg/ml. |
靶标
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功能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
组织特异性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
疾病相关
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
细胞定位
Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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别名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
Immunocytochemistry staining of Cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 (ab7797, diluted 1/400), detected with GAM IgG-Alexa Fluor®488 (diluted 1/200; green), cell nuclei stained with PI (1 µg/ml; orange).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)](https://www.abcam.cn/ps/products/7/ab7797/Images/ab7797-332236-anti-cytokeratin-18-antibody-dc-10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)Immunohistochemistry staining of human colon (paraffin sections) using ab7797 10 μg/ml
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![Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)](https://www.abcam.cn/ps/products/7/ab7797/Images/ab7797-473354-anticytokeratin-18-antibody-dc-10-western-blot.jpg)
Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)All lanes : Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 2 µg/ml
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Non-Reducing)
Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Non-Reducing)
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Reducing)
Lane 4 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Reducing)
Secondary
All lanes : IRDye800-conjugated anti-mouse secondary antibody.A specific band was detected for cytokeratin 18 at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.
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![Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)](https://www.abcam.cn/ps/products/7/ab7797/Images/ab7797-332235-anti-cytokeratin-18-antibody-dc-10-western-blot.jpg)
Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)](https://www.abcam.cn/ps/products/7/ab7797/Images/ab7797-13262-anti-cytokeratin-18-antibody-dc-10-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)Overlay histogram showing MCF-7 cells stained with ab7797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
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![Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)](https://www.abcam.cn/ps/products/7/ab7797/Images/ab7797-505204-anti-cytokeratin-18-antibody-dc-10-flow-cytometry-mcf7-cells-human.jpg)
Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)Flow cytometry analysis (intracellular staining) of MCF-7 cells (red-filled) stained using ab7797 anti-cytokeratin 18 purified antibody (concentration in sample 0,6 μg/ml, GAM APC). Isotype control: Caco-2 cells (black-dashed).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab7797 被引用在 17 文献中.
- Wang F et al. MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells. Anim Nutr 10:319-328 (2022). PubMed: 35891685
- Carmell N et al. Identification and Validation of ERK5 as a DNA Damage Modulating Drug Target in Glioblastoma. Cancers (Basel) 13:N/A (2021). PubMed: 33668183
- Han L et al. Conditionally reprogrammed macaque endocervical cells retain steroid receptor expression and produce mucus. Biol Reprod 102:1191-1202 (2020). PubMed: 32232331
- Al-Khalaf HH et al. Senescent Breast Luminal Cells Promote Carcinogenesis through Interleukin-8-Dependent Activation of Stromal Fibroblasts. Mol Cell Biol 39:N/A (2019). PubMed: 30397077
- Deng H et al. Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures. J Cell Physiol 234:7683-7694 (2019). PubMed: 30609028