Anti-Cytokeratin 18抗体[C-04] (ab668)
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)](https://www.abcam.cn/ps/products/0/ab668/Images/ab668-455403-anti-cytokeratin-18-antibody-flow-cytometry-cervix-human.jpg "Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)")
Key features and details
- Mouse monoclonal [C-04] to Cytokeratin 18
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Human, Mammals
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-Cytokeratin 18抗体[C-04]
参阅全部 Cytokeratin 18 一抗 -
描述
小鼠单克隆抗体[C-04] to Cytokeratin 18 -
宿主
Mouse -
特异性
Human Cytokeratin -
经测试应用
适用于: Flow Cyt (Intra), IHC-Pmore details -
种属反应性
与反应: Human, Mammals -
免疫原
Tissue, cells or virus corresponding to Cytokeratin 18. Cytoskeleton preparation of epidermal carcinoma cell line A431
Database link: P05783 -
阳性对照
- IHC-P: Human skin tissue. Flow Cyt (Intra): HCT 116 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from culture supernatant. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
C-04 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 1 - 4 µg/ml.
Also see PMID 18946470. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-P | (13) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml. Also see PMID 18946470. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
组织特异性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
疾病相关
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
细胞定位
Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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别名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)
Flow cytometric pattern (intracellular staining) of HeLa cells (Human epithelial cell line from cervix adenocarcinoma cell suspension) stained with ab668 (0.6 µg/ml concentration)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)](https://www.abcam.cn/ps/products/0/ab668/Images/ab668-455402-anti-cytokeratin-18-antibody-immunohistochemistry-kidney-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)Immunohistochemistry (paraffin-embedded sections) analysis of human kidney tissue labelling cytokeratin-18 with ab668 at 10µg/ml concentration.
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![Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)](https://www.abcam.cn/ps/products/0/ab668/Images/ab668-455399-anti-cytokeratin-18-antibody-flow-cytometry-hela-cells-human.jpg)
Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)Intracellular flow cytometric analysis (intracellular staining) of HeLa cells (human epithelial cell line from cervix adenocarcinoma cell suspension)stained with 0.6µg/ml ab668 (in red), and unstained HeLa cells (black).
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![Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)](https://www.abcam.cn/ps/products/0/ab668/Images/ab668-3603-anti-cytokeratin-18-antibody-c-04-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)Overlay histogram showing HCT 116 (Human colorectal carcinoma cell line) cells stained with ab668 (red line).
The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab668, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT 116 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (126)
ab668 被引用在 126 文献中.
- Chumduri C et al. Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia. Nat Cell Biol 23:184-197 (2021). PubMed: 33462395
- Hee Hong D et al. Generation of Hepatic Progenitor Cells from the Primary Hepatocytes of Nonhuman Primates Using Small Molecules. Tissue Eng Regen Med 18:305-313 (2021). PubMed: 33591557
- Zheng H et al. Depletion of Toll-Like Receptor-9 Attenuates Renal Tubulointerstitial Fibrosis After Ischemia-Reperfusion Injury. Front Cell Dev Biol 9:641527 (2021). PubMed: 33644078
- Kurdi BA et al. Use of conditioned media (CM) and xeno-free serum substitute on human adipose-derived stem cells (ADSCs) differentiation into urothelial-like cells. PeerJ 9:e10890 (2021). PubMed: 33850639
- Yanagida A et al. Naive stem cell blastocyst model captures human embryo lineage segregation. Cell Stem Cell 28:1016-1022.e4 (2021). PubMed: 33957081