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重组RabMAb

重组Anti-Cytokeratin 17抗体[EP1623] - Cytoskeleton Marker (ab109725)

Submit a review Q&A (1)References (32)
Western blot - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
  • Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1623] to Cytokeratin 17 - Cytoskeleton Marker
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Biotin Carrier Free PE

概述

  • 产品名称

    Anti-Cytokeratin 17抗体[EP1623] - Cytoskeleton Marker
    参阅全部 Cytokeratin 17 一抗

  • 描述

    兔单克隆抗体[EP1623] to Cytokeratin 17 - Cytoskeleton Marker

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    不适用于: IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • A431 and HACAT cell lysates Human squamous cervical carcinoma tissue Human tonsil tissue A431 cells HeLa (human cervix adenocarcinoma) cell lysate

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP1623

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109725于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval
ICC/IF
1/500.

For unpurified use at 1/100 - 1/250.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval
ICC/IF
1/500.

For unpurified use at 1/100 - 1/250.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.

  • 组织特异性

    Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).

  • 疾病相关

    Defects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
    Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.
    Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.

  • 序列相似性

    Belongs to the intermediate filament family.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 39.1 antibody
    • CK 17 antibody
    • CK-17 antibody
    • Cytokeratin-17 antibody
    • K17 antibody
    • K1C17_HUMAN antibody
    • Keratin 17 antibody
    • keratin 17 epitope S1 antibody
    • keratin 17 epitope S2 antibody
    • keratin 17 epitope S4 antibody
    • Keratin 17, type I antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 17 antibody
    • keratin, type i cytoskeletal 17 [version 1] antibody
    • Keratin-17 antibody
    • KRT17 antibody
    • PC antibody
    • PC2 antibody
    • PCHC1 antibody
    • type I cytoskeletal 17 antibody
    see all

图片

  • Western blot - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Western blot - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    All lanes : Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HACAT cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling Cytokeratin 17 with purified ab109725 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Flow Cytometry (Intracellular) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

    Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 17 with purified ab109725 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

    Immunohistochemical analysis of paraffin-embedded human squamous cervical carcinoma tissue using ab109725 at 1/100 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109725 at 1/100 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Immunofluorescent staining of A431 cells using ab109725 at 1/100 dilution
  • Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

文献 (32)

发表研究结果有使用 ab109725？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109725 被引用在 32 文献中.

  • Shi TW  et al. Effects of NCSTN Mutation on Hair Follicle Components in Mice. Dermatology 239:60-71 (2023). PubMed: 35843211
  • Wong HY  et al. Epidermal mutation accumulation in photodamaged skin is associated with skin cancer burden and can be targeted through ablative therapy. Sci Adv 9:eadf2384 (2023). PubMed: 37163607
  • Lozar T  et al. Stress Keratin 17 Is a Predictive Biomarker Inversely Associated with Response to Immune Check-Point Blockade in Head and Neck Squamous Cell Carcinomas and Beyond. Cancers (Basel) 15:N/A (2023). PubMed: 37835599
  • Llorente A  et al. MAF amplification licenses ERα through epigenetic remodelling to drive breast cancer metastasis. Nat Cell Biol 25:1833-1847 (2023). PubMed: 37945904
  • Li Z  et al. ESR1 mutant breast cancers show elevated basal cytokeratins and immune activation. Nat Commun 13:2011 (2022). PubMed: 35440136
View all Publications for this product

客户评价及客户问答

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Question

To whom it may concern,
I bought your Cytokeratin 17 antibody [EP1623] (ab109725) antibody. I could see on your website good pictures of IHC-P. However, it's not written the type of antigen retrival performed (Sodium Citrate? TrisEDTA?).
Couls you please provide me with this information?
Thanks for your help,
Best,

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Answer

Thank you for your enquiry regarding ab109725.

We have uses 0.01M Sodium Citrate Buffer (pH 6.0), as described below:

To prepare Antigen Retrieval stock solutions:

- 10X Stock: Dissolve 29.4 g sodium citrate trisodium salt dihydrate (C6H5Na3O 7 2H2O)
in 1 liter of dH2O. Add 5mL Tween-20.

- 1X Working Solution: Mix 200mL 10X stock with 1800mL dH2O; pH to 6.0


If you need any further assistance in the future, please do not hesitate to contact me.

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