Anti-Cytokeratin 15抗体[LHK15] (ab80522)
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 15 antibody [LHK15] (ab80522)](https://www.abcam.cn/ps/products/80/ab80522/Images/ab80522-7-anti-cytokeratin-15-lhk15-antibody-immunocytochemistry-a431-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 15 antibody [LHK15] (ab80522)")
Key features and details
- Mouse monoclonal [LHK15] to Cytokeratin 15
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Cytokeratin 15抗体[LHK15]
参阅全部 Cytokeratin 15 一抗 -
描述
小鼠单克隆抗体[LHK15] to Cytokeratin 15 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Synthetic peptide corresponding to Human Cytokeratin 15 (C terminal).
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阳性对照
- IHC-P: FFPE human breast normal. ICC-IF: A431 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
LHK15 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80522于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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组织特异性
Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate. -
序列相似性
Belongs to the intermediate filament family. - Information by UniProt
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数据库链接
- Entrez Gene: 507464 Cow
- Entrez Gene: 3866 Human
- Entrez Gene: 16665 Mouse
- Entrez Gene: 287700 Rat
- Omim: 148030 Human
- SwissProt: P19012 Human
- SwissProt: Q61414 Mouse
- SwissProt: Q6IFV3 Rat
see all -
别名
- AI528832 antibody
- CK 15 antibody
- CK-15 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 15 antibody [LHK15] (ab80522)
ab80522 staining Cytokeratin 15 in A431 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab80522 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [LHK15] (ab80522)](https://www.abcam.cn/ps/products/80/ab80522/Images/ab80522-281352-ap3401020assy60646Datasheet.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [LHK15] (ab80522)IHC image of Cytokeratin 15 staining in a formalin fixed, paraffin embedded normal human breast tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80522, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [LHK15] (ab80522)](https://www.abcam.cn/ps/products/80/ab80522/Images/ab80522-214923-ab80522ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [LHK15] (ab80522)This image is courtesy of an anonymous Abreviewab80522 staining Cytokeratin 15 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (undiluted) for 10 hours.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 15 antibody [LHK15] (ab80522)](https://www.abcam.cn/ps/products/80/ab80522/Images/Cytokeratin-15-Primary-antibodies-ab80522-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 15 antibody [LHK15] (ab80522)This image is courtesy of an Abreview submitted by Maciej Gagatab8969 staining keratin 15 in rat hair folicles by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.25% Triton X-100 prior to blocking in 1% BSA for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. A TRITC conjugated mouse anti-mouse antibody, diluted 1/85, was used as the secondary.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (32)
ab80522 被引用在 32 文献中.
- Yusupova M et al. Apoptotic dysregulation mediates stem cell competition and tissue regeneration. Nat Commun 14:7547 (2023). PubMed: 37985759
- Liu Y et al. Germline intergenic duplications at Xq26.1 underlie Bazex-Dupré-Christol basal cell carcinoma susceptibility syndrome. Br J Dermatol 187:948-961 (2022). PubMed: 35986704
- Liao G et al. Single-cell transcriptomics provides insights into the origin and microenvironment of human oesophageal high-grade intraepithelial neoplasia. Clin Transl Med 12:e874 (2022). PubMed: 35608199
- Paudel S et al. Chemopreventive efficacy of silibinin against basal cell carcinoma growth and progression in UVB-irradiated Ptch+/- mice. Carcinogenesis 43:557-570 (2022). PubMed: 35184170
- Ryu YC et al. Pyruvate Kinase M2 Promotes Hair Regeneration by Connecting Metabolic and Wnt/β-Catenin Signaling. Pharmaceutics 14:N/A (2022). PubMed: 36559274