Anti-Cytokeratin 14抗体[RCK107] (ab9220)
Key features and details
- Mouse monoclonal [RCK107] to Cytokeratin 14
- Suitable for: IHC-Fr, WB, ICC/IF
- Reacts with: Dog, Human, Pig
- Isotype: IgG1
概述
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产品名称
Anti-Cytokeratin 14抗体[RCK107]
参阅全部 Cytokeratin 14 一抗 -
描述
小鼠单克隆抗体[RCK107] to Cytokeratin 14 -
宿主
Mouse -
特异性
This antibody reacts exclusively with cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia. -
经测试应用
适用于: IHC-Fr, WB, ICC/IFmore details -
种属反应性
与反应: Dog, Human, Pig -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 14. Cytoskeletal preparation of TR146 epithelial cells.
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阳性对照
- cytoskeletal preparation of TR146 epithelial cells, skin tissue lysate (Human)
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常规说明
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology assay.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
Primary antibody说明
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. -
克隆
单克隆 -
克隆编号
RCK107 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-Fr | (2) |
1/100 - 1/200.
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WB |
1/100 - 1/1000.
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-Fr
1/100 - 1/200. |
WB
1/100 - 1/1000. |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. -
组织特异性
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. -
疾病相关
Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy. -
序列相似性
Belongs to the intermediate filament family. -
细胞定位
Cytoplasm. Nucleus. Expressed in both as a filamentous pattern. - Information by UniProt
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数据库链接
- Entrez Gene: 3861 Human
- Omim: 148066 Human
- SwissProt: P02533 Human
- Unigene: 654380 Human
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别名
- CK 14 antibody
- CK-14 antibody
- ck14 antibody
see all
图片
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Immunohistochemical analysis of swine skin tissue labeling Cytokeratin 14 with ab9220. Showing staining of basal cells.
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ICC/IF image of ab9220 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9220, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Anti-Cytokeratin 14 antibody [RCK107] (ab9220) at 4 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunohistochemical analysis of dog skin epidermis tissue labelling Cytokeratin 14 using ab9220. Staining of basal keratinocyte layer is observed.
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Immunohistochemical analysis of dog skin appendages labelling Cytokeratin 14 with ab9220.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (34)
ab9220 被引用在 34 文献中.
- Jhang JF et al. Improved Urothelial Cell Proliferation, Cytoskeleton and Barrier Function Protein Expression in the Patients With Interstitial Cystitis/Bladder Pain Syndrome After Intravesical Platelet-Rich Plasma Injection. Int Neurourol J 26:S57-67 (2022). PubMed: 35073671
- Wu SY et al. Inflammation and Barrier Function Deficits in the Bladder Urothelium of Patients with Chronic Spinal Cord Injury and Recurrent Urinary Tract Infections. Biomedicines 10:N/A (2022). PubMed: 35203430
- Liu R et al. Dormant Nfatc1 reporter-marked basal stem/progenitor cells contribute to mammary lobuloalveoli formation. iScience 25:103982 (2022). PubMed: 35310332
- Cabaço LC et al. RAB3A Regulates Melanin Exocytosis and Transfer Induced by Keratinocyte-Conditioned Medium. JID Innov 2:100139 (2022). PubMed: 36090299
- Bischof J et al. Paired nicking-mediated COL17A1 reframing for junctional epidermolysis bullosa. Mol Ther 30:2680-2692 (2022). PubMed: 35490295